Crucial role of chelatable iron in silver nanoparticles induced DNA damage and cytotoxicity.

ABSTRACT: Damage to mitochondria and subsequent ROS leakage is a commonly accepted mechanism of nanoparticle toxicity. However, malfunction of mitochondria results in generation of superoxide anion radical (O₂^•-), which due to the relatively low chemical reactivity is rather unlikely to cause harmful effects triggered by nanoparticles. We show that treatment of HepG2 cells with silver nanoparticles (AgNPs) resulted in generation of H₂O₂ instead of O₂^•-, as measured by ROS specific mitochondrial probes. Moreover, addition of a selective iron chelator diminished AgNPs toxicity. Altogether these results suggest that O₂^•- generated during NPs induced mitochondrial collapse is rapidly dismutated to H₂O

SUBMITTER: Grzelak A 

PROVIDER: S-EPMC5975067 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature