Ontology highlight
ABSTRACT:
SUBMITTER: Hatano T
PROVIDER: S-EPMC5976186 | biostudies-literature | 2018 Apr
REPOSITORIES: biostudies-literature
Hatano Tomoyuki T Alioto Salvatore S Roscioli Emanuele E Palani Saravanan S Clarke Scott T ST Kamnev Anton A Hernandez-Fernaud Juan Ramon JR Sivashanmugam Lavanya L Chapa-Y-Lazo Bernardo B Jones Alexandra M E AME Robinson Robert C RC Sampath Karuna K Mishima Masanori M McAinsh Andrew D AD Goode Bruce L BL Balasubramanian Mohan K MK
Journal of cell science 20180423 8
Actins are major eukaryotic cytoskeletal proteins, and they are involved in many important cell functions, including cell division, cell polarity, wound healing and muscle contraction. Despite obvious drawbacks, muscle actin, which is easily purified, is used extensively for biochemical studies of the non-muscle actin cytoskeleton. Here, we report a rapid and cost-effective method to purify heterologous actins expressed in the yeast <i>Pichia pastoris</i> Actin is expressed as a fusion with the ...[more]