¹³C-metabolic flux analysis of ethanol-assimilating Saccharomyces cerevisiae for S-adenosyl-L-methionine production.

ABSTRACT:

Background

Saccharomyces cerevisiae is a host for the industrial production of S-adenosyl-L-methionine (SAM), which has been widely used in pharmaceutical and nutritional supplement industries. It has been reported that the intracellular SAM content in S. cerevisiae can be improved by the addition of ethanol during cultivation. However, the metabolic state in ethanol-assimilating S. cerevisiae remains unclear. In this study, ¹³C-metabolic flux analysis (¹³C-MFA) was conducted to investigate the metabolic regulation responsible for the high SAM production from ethanol.

Results

The comparison between the metabolic flux distributions of central carbon metabolism showed that the metabolic flux levels of the tricarboxylic acid cycle and glyoxylate shunt in t

SUBMITTER: Hayakawa K 

PROVIDER: S-EPMC5977476 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature