A porcine model for pathomorphological age assessment of surgically excised skin wounds.

ABSTRACT: A porcine model of second intention wound healing was established and gross and histological changes needed for accurate assessing the age of wounds were determined. Twenty-five pigs were anesthetized before incision of four wounds on each. The wounds were left to heal from 1 h to 35 days when the pigs were euthanized. In 14 pigs, biopsies were sampled from two wounds between days 2 and 18. By histological evaluation the following characteristics were found useful for determining the age of wounds: neutrophil:macrophage ratio, angiogenesis, hyperplasia of fibroblasts, presence of hemosiderophages and granulation tissue. The latter was present from day 4 (n?=?8 wounds, 100%), but by gross evaluation it was not recognized until day 5 (n?=?4 wounds, 100%). From day 4 to 10, the thickness of granulation tissue increased by 1.2?±?2.4 mm/day. The thickness of collagen fibers within granulation tissue increased throughout the study period, and complete epithelization was reached from day 18.

SUBMITTER: Barington K 

PROVIDER: S-EPMC5977753 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature