Dataset Information

Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.

ABSTRACT: Using promoters expressed in nonendosperm tissues to activate target genes in specific plant tissues or organs with very limited expression in the endosperm is an attractive approach in crop transgenic engineering. In this article, five putative nonendosperm tissue-expressed promoters were cloned from the rice genome and designated P_O_s_NETE₁ , P_O_s_NETE₂ , P_O_s_NETE₃ , P_O_s_NETE₄ and P_O_s_NETE₅ . By qualitatively and quantitatively examining GUSplus reporter gene expression in transgenic rice plants, P_O_s_NETE₁ -P_O_s_NETE₅ were all found to be active in the roots, leaves, stems, sheaths and panicles but not in the endosperm of plants at different developmental stages. In addition, P_O_s_NETE₂ , P_O_s_NETE₄ and P_O_s_NETE₅ were also inactive in rice embryos. Among these promoters, P_O_s_NETE₄ and P_O_s_NETE₅ exhibited higher activities in all of the tested tissues, and their activities in stems, leaves, roots and sheaths were higher than or comparable to those of the rice Actin1 promoter. We also progressively monitored the activities of P_O_s_NETE₁ -P_O_s_NETE₅ in two generations of single-copy lines and found that these promoters were stably expressed between generations. Transgenic rice was produced using P_O_s_NETE₄ and P_O_s_NETE₅ to drive a modified Bt gene, mCry1Ab. Bt protein expressed in the tested plants ranged from 1769.4 to 4428.8 ng/g fresh leaves, whereas Bt protein was barely detected in the endosperm. Overall, our study identified five novel nonendosperm tissue-expressed promoters that might be suitable for rice genetic engineering and might reduce potential social concern regarding the safety of GMO crops.

SUBMITTER: Li H

PROVIDER: S-EPMC5978396 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.

Li Hao H Li Juan J Xu Rongfang R Qin Ruiying R Song Fengshun F Li Li L Wei Pengcheng P Yang Jianbo J

Plant biotechnology journal 20171128 6

Using promoters expressed in nonendosperm tissues to activate target genes in specific plant tissues or organs with very limited expression in the endosperm is an attractive approach in crop transgenic engineering. In this article, five putative nonendosperm tissue-expressed promoters were cloned from the rice genome and designated POsNETE1 , POsNETE2 , POsNETE3 , P ...[more]

PMID: 29105251

Similar Datasets

Project description:Circadian clock genes holds tremendous potential for breeding crops better adapted to environmental fluctuations inherent to climate change. Endogenous TOC1 promoter and CCA1 gene from rice were isolated, cloned and mobilized into pCAMBIA1300 vectors and RNAi constructs A, B and C. Embryogenic calli of varying ages derived from mature seeds of Taipei 309 were employed for Agrobacterium-mediated genetic transformation generating T0, T1 and T2 independent transgenic lines were analyzed for over-expression and repression of CCA1 gene along with various morphological traits. Six hundred and thirty two T0 transgenic plants were generated from rice calli using constructs A, B and C. T0 progeny plants derived from constructs A, B and C did not show any considerable difference in morphological traits. T1and T2 progeny plants derived from construct A exhibited over-expression of CCA1 gene, on the contrary, progeny plants derived from RNAi constructs B and C exhibited repression of CCA1 gene in qRT-PCR analysis at different time points and showed rhythmicity peaking at dawn (6:00 AM) and lowest expression at 12:00 Noon. T1 and T2 progeny plants derived from construct A, namely, A-17 and A-45 exhibited reduced number of tillers/panicles (6-8), reduced thousand seed weight (10.1-16.6g), decreased seed length (4.98 to 6.58mm), decreased seed width (1.1-1.8mm) as compared to wild type plants. T1 and T2 progeny plants of construct B and C showed increased number of tillers/panicles (8-19), better seed yield (4.98-28.9g), increased thousand seed weight (15.6-29.03g), slightly increased seed length (5.7-7.43mm) and slightly increased seed width (1.7-2.98mm) as compared to wild type plants. Chlorophyll content in T1 and T2 progeny plants did not show any significant difference among the three constructs, however, rhythmicity was observed over the period of time in conjunction to CCA1 gene expression. Evidence has been presented which demonstrates that endogenous repression of CCA1 gene resulted in improved morphological traits: increased number of tillers/panicle, thousand seed weight, seed size; whereas, over-expression leads to diminution in morphological traits: decreased number of tillers/panicle, thousand seed weight, seed size as compared to the wild type in T1 and T2 progeny plants. This is first report of successful regulation of endogenous CCA1 gene under control of TOC1 promoter and its effect on improved growth vigor in Japonica rice.

Dataset Information

Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.

Publications

Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets