Unknown

Dataset Information

0

TASK-3 Downregulation Triggers Cellular Senescence and Growth Inhibition in Breast Cancer Cell Lines.


ABSTRACT: TASK-3 potassium channels are believed to promote proliferation and survival of cancer cells, in part, by augmenting their resistance to both hypoxia and serum deprivation. While overexpression of TASK-3 is frequently observed in cancers, the understanding of its role and regulation during tumorigenesis remains incomplete. Here, we evaluated the effect of reducing the expression of TASK-3 in MDA-MB-231 and MCF-10F human mammary epithelial cell lines through small hairpin RNA (shRNA)-mediated knockdown. Our results show that knocking down TASK-3 in fully transformed MDA-MB-231 cells reduces proliferation, which was accompanied by an induction of cellular senescence and cell cycle arrest, with an upregulation of cyclin-dependent kinase (CDK) inhibitors p21 and p27. In non-tumorigenic MCF-10F cells, however, TASK-3 downregulation did not lead to senescence induction, although cell proliferation was impaired and an upregulation of CDK inhibitors was also evident. Our observations implicate TASK-3 as a critical factor in cell cycle progression and corroborate its potential as a therapeutic target in breast cancer treatment.

SUBMITTER: Zuniga R 

PROVIDER: S-EPMC5979529 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

TASK-3 Downregulation Triggers Cellular Senescence and Growth Inhibition in Breast Cancer Cell Lines.

Zúñiga Rafael R   Valenzuela Claudio C   Concha Guierdy G   Brown Nelson N   Zúñiga Leandro L  

International journal of molecular sciences 20180329 4


TASK-3 potassium channels are believed to promote proliferation and survival of cancer cells, in part, by augmenting their resistance to both hypoxia and serum deprivation. While overexpression of TASK-3 is frequently observed in cancers, the understanding of its role and regulation during tumorigenesis remains incomplete. Here, we evaluated the effect of reducing the expression of TASK-3 in MDA-MB-231 and MCF-10F human mammary epithelial cell lines through small hairpin RNA (shRNA)-mediated kno  ...[more]

Similar Datasets

| S-EPMC6209942 | biostudies-literature
| S-EPMC7701500 | biostudies-literature
| S-EPMC5556382 | biostudies-literature
| S-EPMC6333431 | biostudies-literature
| S-EPMC8333348 | biostudies-literature
| S-EPMC3203860 | biostudies-other
| S-EPMC8430780 | biostudies-literature
| S-EPMC4039485 | biostudies-literature
| S-EPMC3017654 | biostudies-literature
2015-06-05 | E-GEOD-38466 | biostudies-arrayexpress