Humanization of fibroblast growth factor 1 single-chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells.
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ABSTRACT:
SUBMITTER: He XX
PROVIDER: S-EPMC5980129 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
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Humanization of fibroblast growth factor 1 single-chain antibody and validation for its antitumorigenic efficacy in breast cancer and glioma cells.
He Xiao-Xiao XX Du Shuang S Gao Shi-Qian SQ Chen Jing-Ying JY Cao Ran-Juan RJ Xing Zhen-Kai ZK Kazim Alia Rizvi Syeda ARS Yu Hua-Li HL Zheng Qing-Chuan QC Zhu Xiao-Juan XJ
Journal of cellular and molecular medicine 20180324 6
Single-chain variable fragment (scFv) antibodies are the smallest immunoglobulins with high antigen-binding affinity. We have previously reported that fibroblast growth factor 1 played pivotal roles in cancer development and generated a mouse scFv (mscFv1C9) could effectively prohibit cancer cell proliferation in vitro and in vivo. Here, we further humanized this scFv (hscFv1C9) using a structure-guided complementarity determining region grafting strategy. The purified hscFv1C9 maintained simila ...[more]
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