The Interaction of CD97/ADGRE5 With ?-Catenin in Adherens Junctions Is Lost During Colorectal Carcinogenesis.

ABSTRACT: The adhesion G-protein-coupled receptor CD97/ADGRE5 is present in adherens junctions of human normal intestinal cells and upregulated in colorectal carcinomas. Here, we examined whether CD97 directly interacts with junctional proteins in normal and malignant colorectal tissue. We identified an association of CD97 with ?-catenin using a proximity ligation assay and confirmed the interaction between both endogenous proteins at the biochemical level by co-immunoprecipitation in human and mouse tissues and cell lines. Glutathione S-transferase-pulldown revealed that CD97 binds ?-catenin through its seven-span transmembrane/intracellular domain(s). To study tumor-associated changes in the interaction of CD97 and ?-catenin in situ, we quantified and correlated both proteins at the membrane, and in the cytoplasm and nuclei of colorectal carcinomas and their corresponding normal tissues (n?=?111). In normal colon, membranous levels of CD97 and ?-catenin correlated strongly (p?

SUBMITTER: Hilbig D 

PROVIDER: S-EPMC5980956 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature