Ontology highlight
ABSTRACT:
SUBMITTER: Czerniecki SM
PROVIDER: S-EPMC5984728 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Czerniecki Stefan M SM Cruz Nelly M NM Harder Jennifer L JL Menon Rajasree R Annis James J Otto Edgar A EA Gulieva Ramila E RE Islas Laura V LV Kim Yong Kyun YK Tran Linh M LM Martins Timothy J TJ Pippin Jeffrey W JW Fu Hongxia H Kretzler Matthias M Shankland Stuart J SJ Himmelfarb Jonathan J Moon Randall T RT Paragas Neal N Freedman Benjamin S BS
Cell stem cell 20180517 6
Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and phenotyping of human kidney organoids. The entire 21-day protocol, from plating to differentiation to analysis, can be performed automatically by liquid-handling robots, or alterna ...[more]