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Physiological and Molecular Characterization of Cephaleuros virescens Occurring in Mango Trees.

ABSTRACT: The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated that the algae is Cephaleuros virescens. The algae must be isolated primarily in liquid medium for further pricking into agar medium. The highest mycelial growth average in Petri dishes occurred when the algae were grown in Trebouxia and BBM. Trebouxia enabled larger cells in the filamentous cells when compared to other culture media.

SUBMITTER: Vasconcelos CV

PROVIDER: S-EPMC5985641 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Physiological and Molecular Characterization of <i>Cephaleuros virescens</i> Occurring in Mango Trees.

Vasconcelos Camila Vilela CV Pereira Fabíola Teodoro FT Duarte Elizabeth Amélia Alves EAA de Oliveira Thiago Alves Santos TAS Peixoto Nei N Carvalho Daniel Diego Costa DDC

The plant pathology journal 20180601 3

The objective of this work was to accomplish the isolation, molecular identification and characterizing the physiology of the causal agent of the algal spot in mango trees. For this purpose, the pathogen growth was assessed in different culture media, with subsequent observation and measurements of the filamentous cells. The molecular identification was made using mycelium obtained from leaf lesions and pure algae colonies grown in culture medium. Descriptions based on DNA sequencing indicated t ...[more]

PMID: 29887771

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