Ontology highlight
ABSTRACT:
SUBMITTER: Haimon Z
PROVIDER: S-EPMC5986066 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Haimon Zhana Z Volaski Alon A Orthgiess Johannes J Boura-Halfon Sigalit S Varol Diana D Shemer Anat A Yona Simon S Zuckerman Binyamin B David Eyal E Chappell-Maor Louise L Bechmann Ingo I Gericke Martin M Ulitsky Igor I Jung Steffen S
Nature immunology 20180518 6
Transcriptome profiling is widely used to infer functional states of specific cell types, as well as their responses to stimuli, to define contributions to physiology and pathophysiology. Focusing on microglia, the brain's macrophages, we report here a side-by-side comparison of classical cell-sorting-based transcriptome sequencing and the 'RiboTag' method, which avoids cell retrieval from tissue context and yields translatome sequencing information. Conventional whole-cell microglial transcript ...[more]