Unknown

Dataset Information

0

Myosin IIa Promotes Antibody Responses by Regulating B Cell Activation, Acquisition of Antigen, and Proliferation.


ABSTRACT: B cell responses are regulated by antigen acquisition, processing, and presentation to helper T cells. These functions are thought to depend on contractile activity of non-muscle myosin IIa. Here, we show that B cell-specific deletion of the myosin IIa heavy chain reduced the numbers of bone marrow B cell precursors and splenic marginal zone, peritoneal B1b, and germinal center B cells. In addition, myosin IIa-deficient follicular B cells acquired an activated phenotype and were less efficient in chemokinesis and extraction of membrane-presented antigens. Moreover, myosin IIa was indispensable for cytokinesis. Consequently, mice with myosin IIa-deficient B cells harbored reduced serum immunoglobulin levels and did not mount robust antibody responses when immunized. Altogether, these data indicate that myosin IIa is a negative regulator of B cell activation but a positive regulator of antigen acquisition from antigen-presenting cells and that myosin IIa is essential for B cell development, proliferation, and antibody responses.

SUBMITTER: Hoogeboom R 

PROVIDER: S-EPMC5986709 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Myosin IIa Promotes Antibody Responses by Regulating B Cell Activation, Acquisition of Antigen, and Proliferation.

Hoogeboom Robbert R   Natkanski Elizabeth M EM   Nowosad Carla R CR   Malinova Dessislava D   Menon Rajesh P RP   Casal Antonio A   Tolar Pavel P  

Cell reports 20180501 8


B cell responses are regulated by antigen acquisition, processing, and presentation to helper T cells. These functions are thought to depend on contractile activity of non-muscle myosin IIa. Here, we show that B cell-specific deletion of the myosin IIa heavy chain reduced the numbers of bone marrow B cell precursors and splenic marginal zone, peritoneal B1b, and germinal center B cells. In addition, myosin IIa-deficient follicular B cells acquired an activated phenotype and were less efficient i  ...[more]

Similar Datasets

| S-EPMC2719775 | biostudies-literature
| S-EPMC266780 | biostudies-literature
| S-EPMC5584216 | biostudies-literature
| S-EPMC3227520 | biostudies-literature
2018-04-14 | GSE113114 | GEO
| S-EPMC2816186 | biostudies-literature
| S-EPMC6724514 | biostudies-literature
| S-EPMC5665442 | biostudies-literature
| S-EPMC3924328 | biostudies-literature
| S-EPMC3228501 | biostudies-literature