Dataset Information

Comparison of three approaches for defining nucleus pulposus and annulus fibrosus on sagittal magnetic resonance images of the lumbar spine.

ABSTRACT:

Objective

To compare three methods commonly used in the literature to define intervertebral disc nucleus pulposus (NP) and annulus fibrosus (AF) on magnetic resonance (MR) images.

Methods

Fifty-two patients (26 males and 26 females; age range, 23-76 years) were recruited for this study; they underwent standard T1/T2-weighted MR imaging, and T2 and T1rho mapping acquisitions. The corresponding midsagittal images were analysed and a total of 256 discs were evaluated, using three different region-of-interest (ROI) drawing methods: (1) radiologist-guided manual ROI (M-ROI); (2) five square ROIs where each measured 20% of the midline disc diameter (5-ROI); and (3) seven square ROIs placed horizontally from anterior to posterior (7-ROI) to define NP and AF. The agreement between the three ROI methods was assessed using intraclass correlation coefficient values and Bland-Altman plots.

Results

Inner AF and NP could not be differentiated on T1/T2-weighted MR imaging, T2 maps, or T1rho maps. The intraclass correlation coefficient values were all > 0.75 when comparing the 5-/7-ROI methods with the M-ROI methods for NP, and 0.167-0.488 for AF when comparing the 7-ROI method with the M-ROI method. The intraclass correlation coefficient values for AF increased to 0.378-0.582 for the M-ROI method compared with the 5-ROI method. Comparable results were obtained with Bland-Altman plots.

Conclusion

The 5-/7-ROI methods agreed with the M-ROI approach for NP selection, while the agreement with AF was moderate to poor, with the 5-ROI method showing slight advantage over the 7-ROI method. Cautions should be taken to interpret the MR relaxometry findings when 5-/7-ROI methods are used to select AF.

SUBMITTER: Mok GSP

PROVIDER: S-EPMC5987025 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Publications

Comparison of three approaches for defining nucleus pulposus and annulus fibrosus on sagittal magnetic resonance images of the lumbar spine.

Mok Greta S P GSP Zhang Duo D Chen Shu-Zhong SZ Yuan Jing J Griffith James F JF Wang Yi Xiang J YXJ

Journal of orthopaedic translation 20160422

<h4>Objective</h4>To compare three methods commonly used in the literature to define intervertebral disc nucleus pulposus (NP) and annulus fibrosus (AF) on magnetic resonance (MR) images.<h4>Methods</h4>Fifty-two patients (26 males and 26 females; age range, 23-76 years) were recruited for this study; they underwent standard T1/T2-weighted MR imaging, and T2 and T1rho mapping acquisitions. The corresponding midsagittal images were analysed and a total of 256 discs were evaluated, using three dif ...[more]

PMID: 30035081

Similar Datasets

Project description:BACKGROUND:The lifetime prevalence of degenerative disc disease is dramatically high. Numerous investigations on disc degeneration have been performed on cells from annulus fibrosus (AF) and nucleus pulposus (NP) of the intervertebral disc (IVD) in cell culture experiments utilising a broad variety of basal culture media. Although the basal media differ in nutrient formulation, it is not known whether the choice of the basal media itself has an impact on the cell's behaviour in vitro. In this study, we evaluated the most common media used for monolayer expansion of AF and NP cells to set standards for disc cell culture. METHODS:Human AF and NP cells were isolated from cervical discs. Cells were expanded in monolayer until passage P2 using six different common culture media containing alpha-Minimal Essential Medium (alpha-MEM), Dulbecco's Modified Eagle's Medium (DMEM) or Ham's F-12 medium (Ham's F-12) as single medium or in a mixture of two media (alpha/F-12, DMEM/alpha, DMEM/F-12). Cell morphology, cell growth, glycosaminoglycan production and quantitative gene expression of cartilage- and IVD-related markers aggrecan, collagen type II, forkhead box F1 and keratin 18 were analysed. Statistical analysis was performed with two-way ANOVA testing and Bonferroni compensation. RESULTS:AF and NP cells were expandable in all tested media. Both cell types showed similar cell morphology and characteristics of dedifferentiation known for cultured disc cells independently from the media. However, proceeding culture in Ham's F-12 impeded cell growth of both AF and NP cells. Furthermore, the keratin 18 gene expression profile of NP cells was changed in alpha-MEM and Ham's F-12. CONCLUSION:The impact of the different media itself on disc cell's behaviour in vitro was low. However, AF and NP cells were only robust, when DMEM was used as single medium or in a mixture (DMEM/alpha, DMEM/F-12). Therefore, we recommend using these media as standard medium for disc cell culture. Our findings are valuable for the harmonisation of preclinical study results and thereby push the development of cell therapies for clinical treatment of disc degeneration.

Dataset Information

Comparison of three approaches for defining nucleus pulposus and annulus fibrosus on sagittal magnetic resonance images of the lumbar spine.

Objective

Methods

Results

Conclusion

Publications

Comparison of three approaches for defining nucleus pulposus and annulus fibrosus on sagittal magnetic resonance images of the lumbar spine.

Similar Datasets

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