Baseline immune profile by CyTOF can predict response to an investigational adjuvanted vaccine in elderly adults.

ABSTRACT:

Background

Mass cytometry, or CyTOF (Cytometry by Time-of-Flight), permits the simultaneous detection of over 40 phenotypic and functional immune markers in individual cells without the issues of spectral overlap seen in traditional flow cytometry.

Methods

In this study, we applied CyTOF to comprehensively characterize the circulating immune cell populations in elderly individuals both before and after administration of an investigational adjuvanted protein vaccine against respiratory syncytial virus (RSV) in a Phase 1a trial. Antigen-specific T cell responses to RSV by IFNγ ELISPOT had been observed in most but not all recipients in the highest dose cohort in this trial. Here, CyTOF was used to characterize the cellular response profile of ELISPOT responders and non-respond

SUBMITTER: Lingblom CMD 

PROVIDER: S-EPMC5987461 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature