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Top-down proteomic characterization of DAOY medulloblastoma tumor cell line.

ABSTRACT: The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike bottom-up ones, allows identifying proteins and peptides in their intact/native forms, disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that characterize DAOY cells.

SUBMITTER: Martelli C

PROVIDER: S-EPMC5988510 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Top-down proteomic characterization of DAOY medulloblastoma tumor cell line.

Martelli Claudia C D'Angelo Luca L Barba Marta M Baranzini Mirko M Inserra Ilaria I Iavarone Federica F Vincenzoni Federica F Tamburrini Gianpiero G Massimi Luca L Rocco Concezio Di CD Caldarelli Massimo M Messana Irene I Michetti Fabrizio F Castagnola Massimo M Lattanzi Wanda W Desiderio Claudia C

EuPA open proteomics 20160401

The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike bottom-up ones, allows identifying proteins and peptides in their intact/native forms, disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and struct ...[more]

PMID: 29900115

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