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SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.

ABSTRACT: We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that reflect the transient physiological state of single cells. These data provide unique insights into the regulatory network of messenger RNA from the nucleus toward the cytoplasm at the single-cell level.

SUBMITTER: Abdelmoez MN

PROVIDER: S-EPMC5989370 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology.

Abdelmoez Mahmoud N MN Iida Kei K Oguchi Yusuke Y Nishikii Hidekazu H Yokokawa Ryuji R Kotera Hidetoshi H Uemura Sotaro S Santiago Juan G JG Shintaku Hirofumi H

Genome biology 20180606 1

We report a microfluidic system that physically separates nuclear RNA (nucRNA) and cytoplasmic RNA (cytRNA) from a single cell and enables single-cell integrated nucRNA and cytRNA-sequencing (SINC-seq). SINC-seq constructs two individual RNA-seq libraries, nucRNA and cytRNA, per cell, quantifies gene expression in the subcellular compartments, and combines them to create novel single-cell RNA-seq data. Leveraging SINC-seq, we discover distinct natures of correlation among cytRNA and nucRNA that ...[more]

PMID: 29871653

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