Dataset Information

Effects of straw return on bacterial communities in a wheat-maize rotation system in the North China Plain.

ABSTRACT: Straw return plays an important role in reducing the use of chemical fertilizer, promoting soil carbon sequestration, thus maintaining soil fertility and alleviating environmental pollution. To examine the effects of straw return on soil bacterial communities, quantitative PCR and high-throughput sequencing approaches were used to analyze the bacterial abundance and community structures at the depths of 5-25 cm and 25-45 cm in the soils under six-year continuous straw return and removal treatments in Langfang, Hebei, the North China Plain. As a result, straw return had no effects on soil chemical properties, bacterial abundance, richness or diversity at both soil depths. In contrast, vertical distributions of available nitrogen and available potassium were affected. Similarly, straw return also changed the vertical distributions of Proteobacteria and Chloroflexi. Principal coordinate analysis based on weighted UniFrac distance matrix indicated a moderate separation of the bacterial community in the soil treated with straw return from that with straw removal at 5-25 cm depth, but they were not distinctly distinguished at 25-45 cm depth. T-test identified increased abundance of Candidatus Latescibacteria in the soil under straw return treatment at 5-25 cm depth but no differentially abundant phyla at 25-45 cm depth was found. These results suggested a selection effect from the six-year continuous straw return treatment and the soil bacterial communities were moderately changed.

SUBMITTER: Yu D

PROVIDER: S-EPMC5991650 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Effects of straw return on bacterial communities in a wheat-maize rotation system in the North China Plain.

Yu Dali D Wen Zhiguo Z Li Xiumei X Song Xiaojun X Wu Huijun H Yang Peilong P

PloS one 20180607 6

Straw return plays an important role in reducing the use of chemical fertilizer, promoting soil carbon sequestration, thus maintaining soil fertility and alleviating environmental pollution. To examine the effects of straw return on soil bacterial communities, quantitative PCR and high-throughput sequencing approaches were used to analyze the bacterial abundance and community structures at the depths of 5-25 cm and 25-45 cm in the soils under six-year continuous straw return and removal treatmen ...[more]

PMID: 29879153

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Project description:Spatial variation of soil respiration (Rs) in cropland ecosystems must be assessed to evaluate the global terrestrial carbon budget. This study aims to explore the spatial characteristics and controlling factors of Rs in a cropland under winter wheat and summer maize rotation in the North China Plain. We collected Rs data from 23 sample plots in the cropland. At the late jointing stage, the daily mean Rs of summer maize (4.74 ?mol CO2 m-2 s-1) was significantly higher than that of winter wheat (3.77?mol CO2 m-2 s-1). However, the spatial variation of Rs in summer maize (coefficient of variation, CV = 12.2%) was lower than that in winter wheat (CV = 18.5%). A similar trend in CV was also observed for environmental factors but not for biotic factors, such as leaf area index, aboveground biomass, and canopy chlorophyll content. Pearson's correlation analyses based on the sampling data revealed that the spatial variation of Rs was poorly explained by the spatial variations of biotic factors, environmental factors, or soil properties alone for winter wheat and summer maize. The similarly non-significant relationship was observed between Rs and the enhanced vegetation index (EVI), which was used as surrogate for plant photosynthesis. EVI was better correlated with field-measured leaf area index than the normalized difference vegetation index and red edge chlorophyll index. All the data from the 23 sample plots were categorized into three clusters based on the cluster analysis of soil carbon/nitrogen and soil organic carbon content. An apparent improvement was observed in the relationship between Rs and EVI in each cluster for both winter wheat and summer maize. The spatial variation of Rs in the cropland under winter wheat and summer maize rotation could be attributed to the differences in spatial variations of soil properties and biotic factors. The results indicate that applying cluster analysis to minimize differences in soil properties among different clusters can improve the role of remote sensing data as a proxy of plant photosynthesis in semi-empirical Rs models and benefit the acquisition of Rs in cropland ecosystems at large scales.

Project description:BACKGROUND:The relative importance of stochasticity versus determinism in soil bacterial communities is unclear, as are the possible influences that alter the balance between these. Here, we investigated the influence of spatial scale on the relative role of stochasticity and determinism in agricultural monocultures consisting only of wheat, thereby minimizing the influence of differences in plant species cover and in cultivation/disturbance regime, extending across a wide range of soils and climates of the North China Plain (NCP). We sampled 243 sites across 1092 km and sequenced the 16S rRNA bacterial gene using MiSeq. We hypothesized that determinism would play a relatively stronger role at the broadest scales, due to the strong influence of climate and soil differences in selecting many distinct OTUs of bacteria adapted to the different environments. In order to test the more general applicability of the hypothesis, we also compared with a natural ecosystem on the Tibetan Plateau. RESULTS:Our results revealed that the relative importance of stochasticity vs. determinism did vary with spatial scale, in the direction predicted. On the North China Plain, stochasticity played a dominant role from 150 to 900 km (separation between pairs of sites) and determinism dominated at more than 900 km (broad scale). On the Tibetan Plateau, determinism played a dominant role from 130 to 1200 km and stochasticity dominated at less than 130 km. Among the identifiable deterministic factors, soil pH showed the strongest influence on soil bacterial community structure and diversity across the North China Plain. Together, 23.9% of variation in soil microbial community composition could be explained, with environmental factors accounting for 19.7% and spatial parameters 4.1%. CONCLUSIONS:Our findings revealed that (1) stochastic processes are relatively more important on the North China Plain, while deterministic processes are more important on the Tibetan Plateau; (2) soil pH was the major factor in shaping soil bacterial community structure of the North China Plain; and (3) most variation in soil microbial community composition could not be explained with existing environmental and spatial factors. Further studies are needed to dissect the influence of stochastic factors (e.g., mutations or extinctions) on soil microbial community distribution, which might make it easier to predictably manipulate the microbial community to produce better yield and soil sustainability outcomes.

Project description:Background: Responses to hypoxia have been investigated in many species; however, comparative study between conspecific geographical populations in different altitude regions is rare, especially for invertebrates . The migratory locust, Locusta migratoria, is widely distributed both on high-altitude Tibetan Plateau (TP) and on low-altitude North China Plain (NP). TP locusts have inhabited Tibetan Plateau since Quaternary glaciations events and thus probably have evolved superior capacity to deal with hypoxia. Results: Here we compared the hypoxic responses of TP and NP locusts from morphological, behavioral and physiological perspectives. We found that TP locusts were more tolerant of extreme hypoxia than NP locusts, with a lower proportion exhibiting stupor, a faster recovery time, and higher respiration rates. We compared the transcriptional profiles of field TP and NP locusts and found that their differences were possibly attributed to a combination of multiple factors, e.g. oxygen, UV radiation, temperature and nutrition. To evaluate why TP locusts respond to extreme hypoxia differently from NP locusts, we subjected them to extreme hypoxia and compared their transcriptional responses. We found that the aerobic metabolism was more active in TP locusts than in NP locusts. RNAi disruption of PDHE1b, an entry gene from glycolysis to TCA cycle, increased the ratio of stupor in Tibetan locusts and decreased the ATP content of Tibetan locusts in hypoxia, confirming the significant importance of this metabolic branch for TP locusts to conquer hypoxia. Conclusions: Here we show that TP locusts are better tolerant of hypoxia than NP locusts and the better capacity to modulate primary metabolism in TP locusts contributes to their superior tolerance of hypoxia compared to NP locusts. FIELD POPULATION: TP locusts vs. NP locusts;direct comparison on 6 separate microarrays; each microarray compares one biological replicate; each biological replicate contains 10 individuals. LAB POPULATION: hypoxia-treated TP locusts vs TP locusts in normoxia; hypoxia-treated NP locusts vs NP locusts in normoxia; direct comparison on 6 separate microarrays; each microarray compares one biological replicate; each biological replicate contains 10 individuals.

Dataset Information

Effects of straw return on bacterial communities in a wheat-maize rotation system in the North China Plain.

Publications

Effects of straw return on bacterial communities in a wheat-maize rotation system in the North China Plain.

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