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Triage of high-risk HPV-positive women in population-based screening by miRNA expression analysis in cervical scrapes; a feasibility study.


ABSTRACT: Background:Primary testing for high-risk HPV (hrHPV) is increasingly implemented in cervical cancer screening programs. Many hrHPV-positive women, however, harbor clinically irrelevant infections, demanding additional disease markers to prevent over-referral and over-treatment. Most promising biomarkers reflect molecular events relevant to the disease process that can be measured objectively in small amounts of clinical material, such as miRNAs. We previously identified eight miRNAs with altered expression in cervical precancer and cancer due to either methylation-mediated silencing or chromosomal alterations. In this study, we evaluated the clinical value of these eight miRNAs on cervical scrapes to triage hrHPV-positive women in cervical screening. Results:Expression levels of the eight candidate miRNAs in cervical tissue samples (n?=?58) and hrHPV-positive cervical scrapes from a screening population (n?=?187) and cancer patients (n?=?38) were verified by quantitative RT-PCR. In tissue samples, all miRNAs were significantly differentially expressed (p?

SUBMITTER: Babion I 

PROVIDER: S-EPMC5992707 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Triage of high-risk HPV-positive women in population-based screening by miRNA expression analysis in cervical scrapes; a feasibility study.

Babion Iris I   Snoek Barbara C BC   Novianti Putri W PW   Jaspers Annelieke A   van Trommel Nienke N   Heideman Daniëlle A M DAM   Meijer Chris J L M CJLM   Snijders Peter J F PJF   Steenbergen Renske D M RDM   Wilting Saskia M SM  

Clinical epigenetics 20180607


<h4>Background</h4>Primary testing for high-risk HPV (hrHPV) is increasingly implemented in cervical cancer screening programs. Many hrHPV-positive women, however, harbor clinically irrelevant infections, demanding additional disease markers to prevent over-referral and over-treatment. Most promising biomarkers reflect molecular events relevant to the disease process that can be measured objectively in small amounts of clinical material, such as miRNAs. We previously identified eight miRNAs with  ...[more]

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