Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation.
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ABSTRACT: Objective
This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay.Results
We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay.
SUBMITTER: Sigar IM
PROVIDER: S-EPMC5992720 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Publications
Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation.
Sigar Ira M IM Schripsema Justin H JH Kelly Kathleen A KA Murthy Ashlesh K AK Manam Srikanth S Ramsey Kyle H KH
BMC research notes 20180607 1
<h4>Objective</h4>This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay.<h4>Results</h4>We have described two methods of eliminating Mycoplasma contamination from Chlamydia laboratory stocks. We conclude that Mycoplasma species commonly contaminating chlamydial stocks do not survive passage in mice. Chlamydia may also be derived Mycoplasma-free by plaque assay. ...[more]
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