Ontology highlight
ABSTRACT:
SUBMITTER: Iqbal ES
PROVIDER: S-EPMC5993425 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Organic & biomolecular chemistry 20180201 7
The ability to incorporate non-canonical amino acids (ncAA) using translation offers researchers the ability to extend the functionality of proteins and peptides for many applications including synthetic biology, biophysical and structural studies, and discovery of novel ligands. Here we describe the high promiscuity of an editing-deficient valine-tRNA synthetase (ValRS T222P). Using this enzyme, we demonstrate ribosomal translation of 11 ncAAs including those with novel side chains, α,α-disubst ...[more]