Dataset Information

High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans.

ABSTRACT: Adherence capacity is one of the principal virulence factors of Streptococcus mutans, and adhesion virulence factors are controlled by small RNAs (sRNAs) at the post-transcriptional level in various bacteria. Here, we aimed to identify and decipher putative adhesion-related sRNAs in clinical strains of S. mutans.RNA deep-sequencing was performed to identify potential sRNAs under different adhesion conditions. The expression of sRNAs was analysed by quantitative real-time PCR (qRT-PCR), and bioinformatic methods were used to predict the functional characteristics of sRNAs.A total of 736 differentially expressed candidate sRNAs were predicted, and these included 352 sRNAs located on the antisense to mRNA (AM) and 384 sRNAs in intergenic regions (IGRs). The top 7 differentially expressed sRNAs were successfully validated by qRT-PCR in UA159, and 2 of these were further confirmed in 100 clinical isolates. Moreover, the sequences of two sRNAs were conserved in other Streptococcus species, indicating a conserved role in such closely related species. A good correlation between the expression of sRNAs and the adhesion of 100 clinical strains was observed, which, combined with GO and KEGG, provides a perspective for the comprehension of sRNA function annotation.This study revealed a multitude of novel putative adhesion-related sRNAs in S. mutans and contributed to a better understanding of information concerning the transcriptional regulation of adhesion in S. mutans.

SUBMITTER: Zhu W

PROVIDER: S-EPMC5994696 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans.

Zhu Wenhui W Liu Shanshan S Liu Jia J Zhou Yan Y Lin Huancai H

Journal of medical microbiology 20180329 5

<h4>Purpose</h4>Adherence capacity is one of the principal virulence factors of Streptococcus mutans, and adhesion virulence factors are controlled by small RNAs (sRNAs) at the post-transcriptional level in various bacteria. Here, we aimed to identify and decipher putative adhesion-related sRNAs in clinical strains of S. mutans.<h4>Methodology</h4>RNA deep-sequencing was performed to identify potential sRNAs under different adhesion conditions. The expression of sRNAs was analysed by quantitativ ...[more]

PMID: 29595415

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Dataset Information

High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans.

Publications

High-throughput sequencing identification and characterization of potentially adhesion-related small RNAs in Streptococcus mutans.

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