Dataset Information

Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy).

ABSTRACT: The surveillance activities for abnormal bivalve mortality events in Italy include the diagnosis of ostreid herpesvirus type 1 (OsHV-1) in symptomatic oysters. OsHV-1-positive oysters (Crassostrea gigas) were used as a source for in vivo virus propagation and a virus-rich sample was selected to perform shotgun sequencing based on Illumina technology. Starting from this unpurified supernatant sample from gills and mantle, we generated 3.5?million reads (2×300?bp) and de novo assembled the whole genome of an Italian OsHV-1 microvariant (OsHV-1-PT). The OsHV-1-PT genome encodes 125 putative ORFs, 7 of which had not previously been predicted in other sequenced Malacoherpesviridae. Overall, OsHV-1-PT displays typical microvariant OsHV-1 genome features, while few polymorphisms (0.08?%) determine its uniqueness. As little is known about the genetic determinants of OsHV-1 virulence, comparing complete OsHV-1 genomes supports a better understanding of the virus pathogenicity and provides new insights into virus-host interactions.

SUBMITTER: Abbadi M

PROVIDER: S-EPMC5994699 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy).

Abbadi Miriam M Zamperin Gianpiero G Gastaldelli Michele M Pascoli Francesco F Rosani Umberto U Milani Adelaide A Schivo Alessia A Rossetti Emanuele E Turolla Edoardo E Gennari Lorenzo L Toffan Anna A Arcangeli Giuseppe G Venier Paola P

The Journal of general virology 20180326 5

The surveillance activities for abnormal bivalve mortality events in Italy include the diagnosis of ostreid herpesvirus type 1 (OsHV-1) in symptomatic oysters. OsHV-1-positive oysters (Crassostrea gigas) were used as a source for in vivo virus propagation and a virus-rich sample was selected to perform shotgun sequencing based on Illumina technology. Starting from this unpurified supernatant sample from gills and mantle, we generated 3.5 million reads (2×300 bp) and de novo assembled the whole g ...[more]

PMID: 29580370

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Dataset Information

Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy).

Publications

Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy).

Similar Datasets

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