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Deregulation of calcium homeostasis in Bcr-Abl-dependent chronic myeloid leukemia.


ABSTRACT: Chronic myeloid leukemia (CML) results from hematopoietic stem cell transformation by the bcr-abl chimeric oncogene, encoding a 210 kDa protein with constitutive tyrosine kinase activity. In spite of the efficiency of tyrosine kinase inhibitors (TKI; Imatinib), other strategies are explored to eliminate CML leukemia stem cells, such as calcium pathways.In this work, we showed that Store-Operated Calcium Entry (SOCE) and thrombin induced calcium influx were decreased in Bcr-Abl expressing 32d cells (32d-p210). The 32d-p210 cells showed modified Orai1/STIM1 ratio and reduced TRPC1 expression that could explain SOCE reduction. Decrease in SOCE and thrombin induced calcium entry was associated to reduced Nuclear Factor of Activated T cells (NFAT) nucleus translocation in 32d-p210 cells. We demonstrated that SOCE blockers enhanced cell mobility of 32d-p210 cells and reduced the proliferation rate in both 32d cell lines. TKI treatment slightly reduced the thrombin-induced response, but imatinib restored SOCE to the wild type level. Bcr-Abl is also known to deregulate Protein Kinase C (PKC), which was described to modulate calcium entries. We showed that PKC enhances SOCE and thrombin induced calcium entries in control cells while this effect is lost in Bcr-Abl-expressing cells.The tyrosine kinase activity seems to regulate calcium entries probably not directly but through a global cellular reorganization involving a PKC pathway. Altogether, calcium entries are deregulated in Bcr-Abl-expressing cells and could represent an interesting therapeutic target in combination with TKI.

SUBMITTER: Cabanas H 

PROVIDER: S-EPMC5995172 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Deregulation of calcium homeostasis in Bcr-Abl-dependent chronic myeloid leukemia.

Cabanas Hélène H   Harnois Thomas T   Magaud Christophe C   Cousin Laëtitia L   Constantin Bruno B   Bourmeyster Nicolas N   Déliot Nadine N  

Oncotarget 20180529 41


<h4>Background</h4>Chronic myeloid leukemia (CML) results from hematopoietic stem cell transformation by the <i>bcr-abl</i> chimeric oncogene, encoding a 210 kDa protein with constitutive tyrosine kinase activity. In spite of the efficiency of tyrosine kinase inhibitors (TKI; Imatinib), other strategies are explored to eliminate CML leukemia stem cells, such as calcium pathways.<h4>Results</h4>In this work, we showed that Store-Operated Calcium Entry (SOCE) and thrombin induced calcium influx we  ...[more]

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