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Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides.

ABSTRACT: A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification by NST, C5-Epi/2-OST and 6-OST1 /6-OST3 , thus resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5-Epi, 2-OST, and 6-OST.

SUBMITTER: Lu W

PROVIDER: S-EPMC5996245 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

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Controlled Chemoenzymatic Synthesis of Heparan Sulfate Oligosaccharides.

Lu Weigang W Zong Chengli C Chopra Pradeep P Pepi Lauren E LE Xu Yongmei Y Amster I Jonathan IJ Liu Jian J Boons Geert-Jan GJ

Angewandte Chemie (International ed. in English) 20180330 19

A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification by NST, C5-Epi/2-OST and 6-OST<sub>1</sub> /6-OST<sub>3</sub> , thus resulting in oligosaccharides differing in N/O-sulfation and epimerization pattern. The enzymatic transformations defined fine substrate requ ...[more]

PMID: 29512241

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