Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus.
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ABSTRACT:
SUBMITTER: Yue XJ
PROVIDER: S-EPMC5999154 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
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Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus.
Yue Xin-Jing XJ Cui Xiao-Wen XW Zhang Zheng Z Hu Wei-Feng WF Li Zhi-Feng ZF Zhang You-Ming YM Li Yue-Zhong YZ
Applied microbiology and biotechnology 20180428 13
Promoter optimization is an economical and effective approach to overexpress heterologous genes and improve the biosynthesis of valuable products. In this study, we swapped the original promoter of the epothilone biosynthetic gene cluster in Myxococcus xanthus with two endogenous strong promoters P <sub>pilA</sub> and P <sub>groEL1</sub> , respectively, which, however, decreased the epothilone production ability. The transcriptional abilities by the two promoters were found to be bloomed in the ...[more]
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