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Transcript variants and expression profiles analysis of Mitf gene in minipigs.


ABSTRACT: Object:To identify transcript variants and expression patterns of porcine Mitf. Materials and methods:A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. Results:Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS. Conclusion:The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF.

SUBMITTER: Guo W 

PROVIDER: S-EPMC6002570 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Transcript variants and expression profiles analysis of <i>Mitf</i> gene in minipigs.

Guo Weiwei W   Ren Lili L   Chen Lei L   Ning Yu Y   Zhao Lidong L   Yang Shiming S  

Journal of otology 20150601 2


<h4>Object</h4>To identify transcript variants and expression patterns of porcine <i>Mitf</i>.<h4>Materials and methods</h4>A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine <i>Mitf</i> gene. Subsequently, 5' RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine <i>Mitf</i> in different tissues.<h4>Results</h4>Four transcript variants of porcine <i>Mitf</i>, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all  ...[more]

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