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Molecular evidence for the occurrence of TYLCV on Mentha longifolia in Jeddah, Saudi Arabia.

ABSTRACT: Begomoviruses are whiteflies transmitted virus causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. Mentha is an important crop being grown here in Saudi Arabia for various purposes. The begomovirus associated disease was observed on Mentha crops during field survey which were growing near to tomato field. There is no published report available about the association of begomovirus on Mentha from this region. So, this work was conducted to identify the causative agent associated with yellow vein mosaic disease. Naturally infected samples were collected from various locations and causative agent was identified by PCR using begomovirus specific primers and further cloned and sequenced bidirectionally. The full genome had total 2785 nucleotides while betasatellite molecule had 1365 nucleotides. Based on full-genome sequence analysis, the identity matrix and phylogenetic analysis showed the highest identity (99.6%) with Tomato yellow leaf curl virus (TYLCV) reported from tomato in the Kingdom of Saudi Arabia. The identified begomovirus was observed as isolate of TYLCV.

SUBMITTER: Sohrab SS

PROVIDER: S-EPMC6003059 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Molecular evidence for the occurrence of TYLCV on <i>Mentha longifolia</i> in Jeddah, Saudi Arabia.

Sohrab Sayed Sartaj SS Daur Ihsanullah I

Virusdisease 20180402 2

Begomoviruses are whiteflies transmitted virus causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. Mentha is an important crop being grown here in Saudi Arabia for various purposes. The begomovirus associated disease was observed on Mentha crops during field survey which were growing near to tomato field. There is no published report available about the association of begomovirus on Mentha from this region. So, this work was cond ...[more]

PMID: 29911154

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Project description:Mentha is a very important crop grown and used extensively for many purposes in the Kingdom of Saudi Arabia. Begomoviruses are whitefly-transmitted viruses causing serious disease in many important plants exhibiting variable symptoms with significant economic loss globally. During farmers' field survey, yellow vein mosaic disease was observed in Mentha longifolia plants growing near tomato fields in Saudi Arabia. The causative agent was identified in 11 out of 19 samples using begomovirus-specific primers and the association of begomovirus with yellow vein mosaic disease in M. longifolia was confirmed. The full-length viral genome and betasatellite were amplified, cloned, and sequenced bidirectionally. The full DNA-A genome was found to have 2785 nucleotides with 1365 bp-associated betasatellite molecule. An attempt was made to amplify DNA-B, but none of the samples produced any positive amplicon of expected size which indicated the presence of monopartite begomovirus. The sequence identity matrix and phylogenetic analysis, based on full genome showed the highest identity (99.6%) with Tomato yellow leaf curl virus (TYLCV) and in phylogenetic analysis it formed a closed cluster with Tomato leaf curl virus infecting tomato and Corchorus crop in Saudi Arabia. The sequence analysis results of betasatellites showed the highest identity (98.9%) with Tomato yellow leaf curl betasatellites infecting tomato and phylogenetic analysis using betasatellites formed a close cluster with Tomato yellow leaf curl betasatellites infecting tomato and Corchorus crops, which has already been reported to cause yellow vein mosaic and leaf curl disease in many cultivated and weed crops growing in Saudi Arabia. The identified begomovirus associated with yellow vein mosaic disease in mentha could be a mutated strain of TYLCV and tentatively designated as TYLCV-Mentha isolate. Based on published data and latest information, this is the first report of identification of Tomato yellow leaf curl virus associated with yellow vein mosaic disease of M. longifolia from Saudi Arabia.

Project description:ObjectivesThis study evaluates awareness of folic acid and neural tube defects, use of folic acid and knowledge of folic acid usage among female university students in Saudi Arabia.MethodsAn analytical cross-sectional survey was conducted in the Jeddah branch of Saudi Electronic University. A total of 400 female students in the 19-45 years age group were contacted to participate in the study using a convenience sampling method. Eighty-eight students refused to participate, so 312 students were included in the study. Demographic characteristics of participants and their awareness, usage and knowledge of folic acid are presented as frequencies and percentages. Logistic regression analysis was used to compute crude odds ratios to show the association between demographic factors and knowledge of folic acid supplementation. A further adjusted odds ratio was calculated to control for other cofactors. A p-value < 0.05 was considered significant.ResultsOverall, 81.1% (253/312) of respondents had heard of folic acid, 30.4% knew about neural tube defects, and 47.1% of respondents reported using folic acid supplements. Marriage was found to be associated with awareness of folic acid (adjusted odds ratio (95% confidence interval) = 2.64 (1.01-6.91)). Health sciences specialization and experience of pregnancy were associated with the usage of folic acid (adjusted odds ratio (95% confidence interval) = 3.46 (1.73-6.91) and adjusted odds ratio (95% confidence interval) = 6.70 (3.09-14.53), respectively). A bachelor's and above education level was found to be associated with knowledge of folic acid deficiency leading to neural tube defects (adjusted odds ratio (95% confidence interval) = 2.03 (1.17-3.52)), and health sciences specialization was found to be associated with knowledge of natural sources of folic acid (adjusted odds ratio (95% confidence interval) = 2.33 (1.25-4.36)). Similarly, marriage was found to be associated with knowledge of the correct dosage of folic acid and timing of folic acid to prevent neural tube defects (adjusted odds ratio (95% confidence interval) = 6.34 (3.50-11.48) and adjusted odds ratio (95% confidence interval) = 3.45 (1.93-6.16), respectively). Experience of pregnancy was found to be an associated with good total knowledge of folic acid (adjusted odds ratio (95% confidence interval) = 3.05 (1.65-5.63); p = 0.0001).ConclusionThe study findings highlight the lack of awareness among female university students about the importance of folic acid usage in the prevention of neural tube defects. There is a need for interventional programs in universities to create awareness.

Dataset Information

Molecular evidence for the occurrence of TYLCV on Mentha longifolia in Jeddah, Saudi Arabia.

Publications

Molecular evidence for the occurrence of TYLCV on <i>Mentha longifolia</i> in Jeddah, Saudi Arabia.

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