Ontology highlight
ABSTRACT:
SUBMITTER: Dart ML
PROVIDER: S-EPMC6004564 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
ACS medicinal chemistry letters 20180416 6
Protein thermal shift assays (TSAs) provide a means for characterizing target engagement through ligand-induced thermal stabilization. Although these assays are widely utilized for screening libraries and validating hits in drug discovery programs, they can impose encumbering operational requirements, such as the availability of purified proteins or selective antibodies. Appending the target protein with a small luciferase (NanoLuc) allows coupling of thermal denaturation with luminescent output ...[more]