Small molecule regulated dynamic structural changes of human G-quadruplexes.

ABSTRACT: The changes in structure and dynamics of oncogenic (c-MYC) and telomeric (h-TELO) G-rich DNA sequences due to the binding of a novel carbazole derivative (BTC) are elucidated using single-molecule Förster resonance energy transfer (sm-FRET), fluorescence correlation spectroscopy (FCS) and NMR spectroscopy. In contrast to the previous reports on the binding of ligands to pre-folded G-quadruplexes, this work illustrates how ligand binding changes the conformational equilibria of both unstructured G-rich DNA sequences and K⁺-folded G-quadruplexes. The results demonstrate that K⁺ free c-MYC and h-TELO exist as unfolded and partially folded conformations. The binding of BTC shifts the equilibria of both investigated DNA sequences t

SUBMITTER: Debnath M 

PROVIDER: S-EPMC6006475 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature