Dataset Information

Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer.

ABSTRACT: The incidence of pancreatic cancer is increasing annually in Asia as a whole. Pancreatic cancer ranks sixth in terms of incidence of all malignant tumors. Circular RNA (circRNA) is a type of non-coding RNA which forms a covalently closed continuous loop. CircRNA is extensively expressed in the cytoplasm, and is markedly conservative and stable. MicroRNA (miR)-378a-3p and human (hsa)_circ_0006215 were detected using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in tissue and cells. Western blot analysis detected the SERPINA4 and hsa_circ_0006215 expression in tissue. A Cell Counting Kit-8 assay was used to determine cell stability. Flow cytometry was used to determine the cell apoptotic rate. Transwell assays were used to determine cell migration. hsa_circ_0006215 was identified as a significantly upregulated circRNA. RT-qPCR results verified that, in 30 samples of pancreatic cancer tissue and paracancerous tissue, hsa_circ_0006215 expression was increased in pancreatic cancer tissue, miR-378a-3p expression was decreased in pancreatic cancer tissue, and SERPINA4 expression was increased in pancreatic cancer tissue (P<0.05). Using bioinformatics database and bioinformatics analysis, the interaction network of hsa_circ_0006215 indicated that this circRNA was most likely to regulate the expression of miR-378a-3p. Further interaction analysis revealed that the SERPINA4 gene was a regulatory target gene most likely to have an influence. The present study identified the effects of hsa_circ_0006215, miR-378a-3p and SERPINA4 signaling pathways in pancreatic cancer cells.

SUBMITTER: Zhu P

PROVIDER: S-EPMC6006498 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer.

Zhu Ping P Ge Nan N Liu Dongyan D Yang Fan F Zhang Kai K Guo Jintao J Liu Xiang X Wang Sheng S Wang Guoxin G Sun Siyu S

Oncology letters 20180507 1

The incidence of pancreatic cancer is increasing annually in Asia as a whole. Pancreatic cancer ranks sixth in terms of incidence of all malignant tumors. Circular RNA (circRNA) is a type of non-coding RNA which forms a covalently closed continuous loop. CircRNA is extensively expressed in the cytoplasm, and is markedly conservative and stable. MicroRNA (miR)-378a-3p and human (hsa)_circ_0006215 were detected using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in tis ...[more]

PMID: 29930719

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Project description:The aim of this study was to investigate the relationships between pulmonary function, respiratory muscle strength, perceived dyspnea, degree of fatigue, and activity of daily living with motor function and neurological status in stroke patients with stable congestive heart failure (CHF).This was a cohort study in a tertiary care medical center. Stroke patients with CHF and exertional dyspnea (New York Heart Association class I-III) were recruited. The baseline characteristics included duration of disease, Brunnstrom stage, spirometry, resting heart rate, resting oxyhemoglobin saturation (SpO2), maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), Borg scale, fatigue scale, and Barthel index.A total of 47 stroke patients (24 males, 23 females, mean age 65.9?±?11.5 years) were included. The average Brunnstrom stages of affected limbs were 3.6?±?1.3 over the proximal parts and 3.5?±?1.4 over the distal parts of upper limbs, and 3.9?±?0.9 over lower limbs. The average forced vital capacity (FVC) was 2.0?±?0.8?L, with a predicted FVC% of 67.9?±?18.8%, forced expiratory volume in the first second (FEV1) of 1.6?±?0.7?L, predicted FEV1% of 70.6?±?20.1%, FEV1/FVC of 84.2?±?10.5%, and maximum mid-expiratory flow of 65.4?±?29.5%. The average MIP and MEP were -52.9?±?33.3?cmH2O and 60.8?±?29.0?cmH2O, respectively. The Borg scale was 1.5?±?0.8. MIP was negatively associated with the average Brunnstrom stage of the proximal (r?=?-0.318, P?<?0.05) and distal (r?=?-0.391, P?<?0.01) parts of the upper extremities and lower extremities (r?=?-0.288, P?<?0.05), FVC (r?=?-0.471, P?<?0.01), predicted FVC% (r?=?-0.299, P?<?0.05), and FEV1 (r?=?-0.397, P?<?0.01). MEP was positively associated with average Brunnstrom stage of the distal area of the upper extremities (r?=?0.351, P?<?0.05), FVC (r?=?0.526, P?<?0.01), FEV1 (r?=?0.429, P?<?0.01), and FEV1/FVC (r?=?-0.482, P?<?0.01). FEV1/FVC was negatively associated with the average Brunnstrom stage over the proximal (r?=?-0.414, P?<?0.01) and distal (r?=?-0.422, P?<?0.01) parts of the upper extremities and lower extremities (r?=?-0.311, P?<?0.05) and Barthel index (r?=?-0.313, P?<?0.05).Stroke patients with stable CHF and exertional dyspnea had restrictive lung disorder and respiratory muscle weakness, which were associated with the neurological status of the affected limbs. FVC was more strongly associated with MIP and MEP than predicted FVC%. FEV1/FVC may be used as a reference for the pulmonary dysfunction.

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Dataset Information

Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer.

Publications

Preliminary investigation of the function of hsa_circ_0006215 in pancreatic cancer.

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