Cationic polymers for non-viral gene delivery to human T cells.
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ABSTRACT:
SUBMITTER: Olden BR
PROVIDER: S-EPMC6008197 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Cationic polymers for non-viral gene delivery to human T cells.
Olden Brynn R BR Cheng Yilong Y Yu Jonathan L JL Pun Suzie H SH
Journal of controlled release : official journal of the Controlled Release Society 20180305
The clinical success of chimeric antigen receptor (CAR) T cell immunotherapy in treating multiple blood cancers has created a need for efficient methods of ex vivo gene delivery to primary human T cells for cell engineering. Here, we synthesize and evaluate a panel of cationic polymers for gene delivery to both cultured and primary human T cells. We show that a subset of comb- and sunflower-shaped pHEMA-g-pDMAEMA polymers can mediate transfection with efficiencies up to 50% in the Jurkat human T ...[more]
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