Ontology highlight
ABSTRACT:
SUBMITTER: Ortiz-Soto ME
PROVIDER: S-EPMC6009436 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Ortiz-Soto Maria Elena ME Ertl Julia J Mut Jürgen J Adelmann Juliane J Le Thien Anh TA Shan Junwen J Teßmar Jörg J Schlosser Andreas A Engels Bernd B Seibel Jürgen J
Chemical science 20180522 24
Carbohydrate processing enzymes are sophisticated tools of living systems that have evolved to execute specific reactions on sugars. Here we present for the first time the site-selective chemical modification of exposed tyrosine residues in SacB, a levansucrase from <i>Bacillus megaterium</i> (<i>Bm</i>-LS) for enzyme engineering purposes <i>via</i> an ene-type reaction. <i>Bm</i>-LS is unable to sustain the synthesis of high molecular weight (HMW) levan (a fructose polymer) due to protein-oligo ...[more]