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Histone deacetylation promotes transcriptional silencing at facultative heterochromatin.


ABSTRACT: It is important to accurately regulate the expression of genes involved in development and environmental response. In the fission yeast Schizosaccharomyces pombe, meiotic genes are tightly repressed during vegetative growth. Despite being embedded in heterochromatin these genes are transcribed and believed to be repressed primarily at the level of RNA. However, the mechanism of facultative heterochromatin formation and the interplay with transcription regulation is not understood. We show genome-wide that HDAC-dependent histone deacetylation is a major determinant in transcriptional silencing of facultative heterochromatin domains. Indeed, mutation of class I/II HDACs leads to increased transcription of meiotic genes and accumulation of their mRNAs. Mechanistic dissection of the pho1 gene where, in response to phosphate, transient facultative heterochromatin is established by overlapping lncRNA transcription shows that the Clr3 HDAC contributes to silencing independently of SHREC, but in an lncRNA-dependent manner. We propose that HDACs promote facultative heterochromatin by establishing alternative transcriptional silencing.

SUBMITTER: Watts BR 

PROVIDER: S-EPMC6009587 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Histone deacetylation promotes transcriptional silencing at facultative heterochromatin.

Watts Beth R BR   Wittmann Sina S   Wery Maxime M   Gautier Camille C   Kus Krzysztof K   Birot Adrien A   Heo Dong-Hyuk DH   Kilchert Cornelia C   Morillon Antonin A   Vasiljeva Lidia L  

Nucleic acids research 20180601 11


It is important to accurately regulate the expression of genes involved in development and environmental response. In the fission yeast Schizosaccharomyces pombe, meiotic genes are tightly repressed during vegetative growth. Despite being embedded in heterochromatin these genes are transcribed and believed to be repressed primarily at the level of RNA. However, the mechanism of facultative heterochromatin formation and the interplay with transcription regulation is not understood. We show genome  ...[more]

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