Ontology highlight
ABSTRACT:
SUBMITTER: Fislage M
PROVIDER: S-EPMC6009598 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Fislage Marcus M Zhang Jingji J Brown Zuben Patrick ZP Mandava Chandra Sekhar CS Sanyal Suparna S Ehrenberg Måns M Frank Joachim J
Nucleic acids research 20180601 11
The GTPase EF-Tu in ternary complex with GTP and aminoacyl-tRNA (aa-tRNA) promotes rapid and accurate delivery of cognate aa-tRNAs to the ribosomal A site. Here we used cryo-EM to study the molecular origins of the accuracy of ribosome-aided recognition of a cognate ternary complex and the accuracy-amplifying role of the monitoring bases A1492, A1493 and G530 of the 16S rRNA. We used the GTPase-deficient EF-Tu variant H84A with native GTP, rather than non-cleavable GTP analogues, to trap a near- ...[more]