Ontology highlight
ABSTRACT:
SUBMITTER: Bennet IA
PROVIDER: S-EPMC6009646 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Bennet Ian A IA Finger L David LD Baxter Nicola J NJ Ambrose Benjamin B Hounslow Andrea M AM Thompson Mark J MJ Exell Jack C JC Shahari Nur Nazihah B Md NNBM Craggs Timothy D TD Waltho Jonathan P JP Grasby Jane A JA
Nucleic acids research 20180601 11
Human flap endonuclease-1 (hFEN1) catalyzes the divalent metal ion-dependent removal of single-stranded DNA protrusions known as flaps during DNA replication and repair. Substrate selectivity involves passage of the 5'-terminus/flap through the arch and recognition of a single nucleotide 3'-flap by the α2-α3 loop. Using NMR spectroscopy, we show that the solution conformation of free and DNA-bound hFEN1 are consistent with crystal structures; however, parts of the arch region and α2-α3 loop are ...[more]