Ontology highlight
ABSTRACT:
SUBMITTER: Missoury S
PROVIDER: S-EPMC6009658 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Missoury Sophia S Plancqueel Stéphane S Li de la Sierra-Gallay Ines I Zhang Wenhua W Liger Dominique D Durand Dominique D Dammak Raoudha R Collinet Bruno B van Tilbeurgh Herman H
Nucleic acids research 20180601 11
The universal N6-threonylcarbamoyladenosine (t6A) modification at position A37 of ANN-decoding tRNAs is essential for translational fidelity. In bacteria the TsaC enzyme first synthesizes an l-threonylcarbamoyladenylate (TC-AMP) intermediate. In cooperation with TsaB and TsaE, TsaD then transfers the l-threonylcarbamoyl-moiety from TC-AMP onto tRNA. We determined the crystal structure of the TsaB-TsaE-TsaD (TsaBDE) complex of Thermotoga maritima in presence of a non-hydrolysable AMPCPP. TsaE is ...[more]