Ontology highlight
ABSTRACT:
SUBMITTER: Frenz B
PROVIDER: S-EPMC6009829 | biostudies-literature | 2017 Aug
REPOSITORIES: biostudies-literature
Frenz Brandon B Walls Alexandra C AC Egelman Edward H EH Veesler David D DiMaio Frank F
Nature methods 20170619 8
Accurate atomic modeling of macromolecular structures into cryo-electron microscopy (cryo-EM) maps is a major challenge, as the moderate resolution makes accurate placement of atoms difficult. We present Rosetta enumerative sampling (RosettaES), an automated tool that uses a fragment-based sampling strategy for de novo model completion of macromolecular structures from cryo-EM density maps at 3-5-Å resolution. On a benchmark set of nine proteins, RosettaES was able to identify near-native confor ...[more]