Unknown

Dataset Information

0

Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens.


ABSTRACT: Tyrosinase is known for an enzyme that plays a key role in producing the initial precursor of melanin biosynthesis. Inhibition of the catalytic reaction of this enzyme led to some advantage such as skin-whitening and anti-insect agents. To find a natural compound with inhibitory activity towards tyrosinase, the five flavonoids of kushenol A (1), 8-prenylkaempferol (2), kushenol C (3), formononetin (4) and 8-prenylnaringenin (5) were isolated by column chromatography from a 95% methanol extract of Sophora flavescens. The ability of these flavonoids to block the conversion of L-tyrosine to L-DOPA by tyrosinase was tested in vitro. Compounds 1 and 2 exhibited potent inhibitory activity, with IC50 values less than 10?µM. Furthermore, enzyme kinetics and molecular docking analysis revealed the formation of a binary encounter complex between compounds 1-4 and the enzyme. Also, all of the isolated compounds (1-5) were confirmed to possess antioxidant activity.

SUBMITTER: Kim JH 

PROVIDER: S-EPMC6009905 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Kushenol A and 8-prenylkaempferol, tyrosinase inhibitors, derived from Sophora flavescens.

Kim Jang Hoon JH   Cho In Sook IS   So Yang Kang YK   Kim Hyeong-Hwan HH   Kim Young Ho YH  

Journal of enzyme inhibition and medicinal chemistry 20181201 1


Tyrosinase is known for an enzyme that plays a key role in producing the initial precursor of melanin biosynthesis. Inhibition of the catalytic reaction of this enzyme led to some advantage such as skin-whitening and anti-insect agents. To find a natural compound with inhibitory activity towards tyrosinase, the five flavonoids of kushenol A (1), 8-prenylkaempferol (2), kushenol C (3), formononetin (4) and 8-prenylnaringenin (5) were isolated by column chromatography from a 95% methanol extract o  ...[more]

Similar Datasets

| S-EPMC10887985 | biostudies-literature
| PRJDB3906 | ENA
| PRJNA892263 | ENA
| PRJNA494446 | ENA
| PRJNA556065 | ENA
| S-EPMC6150336 | biostudies-literature
| S-EPMC7800985 | biostudies-literature
| S-EPMC10465603 | biostudies-literature
| S-EPMC7396426 | biostudies-literature
| S-EPMC6273523 | biostudies-literature