Unknown

Dataset Information

0

Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification.

ABSTRACT: Due to the lowered pKa of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.

SUBMITTER: Ring CM 

PROVIDER: S-EPMC6010304 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification.

Ring Christine M CM   Iqbal Emil S ES   Hacker David E DE   Hartman Matthew C T MCT   Cropp T Ashton TA  

Organic & biomolecular chemistry 20170501 21

Due to the lowered pK<sub>a</sub> of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures. ...[more]

Similar Datasets

Unknown Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids.
| S-EPMC6101111 | biostudies-literature
Unknown Selective purification of the thiol peptides of myosin.
| S-EPMC1198697 | biostudies-other
Unknown Increasing the affinity of selective bZIP-binding peptides through surface residue redesign.
| S-EPMC4088978 | biostudies-literature
Unknown Selective purification of recombinant neuroactive peptides using the flagellar type III secretion system.
| S-EPMC3372961 | biostudies-literature
Unknown Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples.
| S-EPMC5656043 | biostudies-literature
Unknown Human IgA-binding peptides selected from random peptide libraries: affinity maturation and application in IgA purification.
| S-EPMC3522307 | biostudies-literature
Unknown A diagonal electrophoretic method for selective purification of methionine peptides.
| S-EPMC1270284 | biostudies-other
Unknown One-step selective affinity purification and immobilization of His-tagged enzyme by recyclable magnetic nanoparticles.
| S-EPMC8182278 | biostudies-literature
Unknown A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor.
| S-EPMC3973576 | biostudies-literature
Unknown Biomimetic α-selective ribosylation enables two-step modular synthesis of biologically important ADP-ribosylated peptides.
| S-EPMC7645758 | biostudies-literature