Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification.
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ABSTRACT:
SUBMITTER: Ring CM
PROVIDER: S-EPMC6010304 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Publications
Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification.
Ring Christine M CM Iqbal Emil S ES Hacker David E DE Hartman Matthew C T MCT Cropp T Ashton TA
Organic & biomolecular chemistry 20170501 21
Due to the lowered pK<sub>a</sub> of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures. ...[more]
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