Isolation of cells from whole blood using shear-induced diffusion.

ABSTRACT: Extraction of cells of interest directly from whole blood is in high demand, yet extraordinary challenging due to the complex hemodynamics and hemorheology of the sample. Herein, we describe a new microfluidic platform that exploits the intrinsic complex properties of blood for continuous size-selective focusing and separation of cells directly from unprocessed whole blood. The novel system only requires routinely accessible saline solution to form a sandwiched fluid configuration and to initiate a strong effect of shear-induced diffusion of cells, which is coupled with fluid inertia for effective separation. Separations of beads and cells from whole blood have been successfully demonstrated with high efficiency (89.8%) at throughput of 6.75 mL/hr (10⁶-10⁷ cells/s) of

SUBMITTER: Zhou J 

PROVIDER: S-EPMC6010421 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature