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Isolation of cells from whole blood using shear-induced diffusion.


ABSTRACT: Extraction of cells of interest directly from whole blood is in high demand, yet extraordinary challenging due to the complex hemodynamics and hemorheology of the sample. Herein, we describe a new microfluidic platform that exploits the intrinsic complex properties of blood for continuous size-selective focusing and separation of cells directly from unprocessed whole blood. The novel system only requires routinely accessible saline solution to form a sandwiched fluid configuration and to initiate a strong effect of shear-induced diffusion of cells, which is coupled with fluid inertia for effective separation. Separations of beads and cells from whole blood have been successfully demonstrated with high efficiency (89.8%) at throughput of 6.75?mL/hr (106-107 cells/s) of whole blood. Rapid isolation of circulating tumor cells (CTCs) from peripheral blood sample of hepatocarcinoma patients is also shown as a proof of principle.

SUBMITTER: Zhou J 

PROVIDER: S-EPMC6010421 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Isolation of cells from whole blood using shear-induced diffusion.

Zhou Jian J   Tu Chunlong C   Liang Yitao Y   Huang Bobo B   Fang Yifeng Y   Liang Xiao X   Papautsky Ian I   Ye Xuesong X  

Scientific reports 20180620 1


Extraction of cells of interest directly from whole blood is in high demand, yet extraordinary challenging due to the complex hemodynamics and hemorheology of the sample. Herein, we describe a new microfluidic platform that exploits the intrinsic complex properties of blood for continuous size-selective focusing and separation of cells directly from unprocessed whole blood. The novel system only requires routinely accessible saline solution to form a sandwiched fluid configuration and to initiat  ...[more]

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