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Data on horizontally transferred genes in California two-spot octopus, Octopus bimaculoides.

ABSTRACT: Horizontal gene transfer (HGT), a mechanism that shares genetic material between the host and donor from separated offspring branches, has been described as a means of producing novel and beneficial phenotypes for the host organisms. In the present study, 12 HGT genes were identified from California two-spot octopus Octopus bimaculoides based on a similarity search, phylogenetic construction, gene composition analysis and PCR (Polymerase Chain Reaction) validation. The data collected from the HGT genes from octopus, indicating the phylogenetic incongruences, CodonW analysis, PCR products, detailed motifs and organisms used in screening. In phylogenetic screening, those genes were nested within bacteria homologs and identified as HGT genes transferred from the bacteria to the octopus. The motifs were similar in proteins of the horizontally acquired Zn-metalloproteinases, but differed to endogenous proteins. CodonW was employed to investigate the codon usage bias between HGT genes and other genes in the octopus genome. In PCR validation, all the HGT genes could be produced as amplified fragments. The results collectively indicated the existence of HGT in molluscs and its potential l contribution to the evolution of octopus with regards to functional innovation and adaptability.

SUBMITTER: Conghui L

PROVIDER: S-EPMC6011040 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Data on horizontally transferred genes in California two-spot octopus, <i>Octopus bimaculoides</i>.

Conghui Liu L Bo Liu L Yan Zhang Z Fan Jiang J Yuwei Ren R Shuqu Li L Hengchao Wang W Wei Fan F

Data in brief 20180601

Horizontal gene transfer (HGT), a mechanism that shares genetic material between the host and donor from separated offspring branches, has been described as a means of producing novel and beneficial phenotypes for the host organisms. In the present study, 12 HGT genes were identified from California two-spot octopus <i>Octopus bimaculoides</i> based on a similarity search, phylogenetic construction, gene composition analysis and PCR (Polymerase Chain Reaction) validation. The data collected from ...[more]

PMID: 29942828

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Project description:BackgroundHorizontal gene transfer plays an important role in evolution because it sometimes allows recipient lineages to adapt to new ecological niches. High genes transfer frequencies were inferred for prokaryotic and early eukaryotic evolution. Does horizontal gene transfer also impact phylogenetic reconstruction of the evolutionary history of genomes and organisms? The answer to this question depends at least in part on the actual gene transfer frequencies and on the ability to weed out transferred genes from further analyses. Are the detected transfers mainly false positives, or are they the tip of an iceberg of many transfer events most of which go undetected by current methods?ResultsPhylogenetic detection methods appear to be the method of choice to infer gene transfers, especially for ancient transfers and those followed by orthologous replacement. Here we explore how well some of these methods perform using in silico transfers between the terminal branches of a gamma proteobacterial, genome based phylogeny. For the experiments performed here on average the AU test at a 5% significance level detects 90.3% of the transfers and 91% of the exchanges as significant. Using the Robinson-Foulds distance only 57.7% of the exchanges and 60% of the donations were identified as significant. Analyses using bipartition spectra appeared most successful in our test case. The power of detection was on average 97% using a 70% cut-off and 94.2% with 90% cut-off for identifying conflicting bipartitions, while the rate of false positives was below 4.2% and 2.1% for the two cut-offs, respectively. For all methods the detection rates improved when more intervening branches separated donor and recipient.ConclusionRates of detected transfers should not be mistaken for the actual transfer rates; most analyses of gene transfers remain anecdotal. The method and significance level to identify potential gene transfer events represent a trade-off between the frequency of erroneous identification (false positives) and the power to detect actual transfer events.

Project description:BackgroundHorizontal gene transfer (HGT), a source of genetic variation, is generally considered to facilitate hosts' adaptability to environments. However, convincing evidence supporting the significant contribution of the transferred genes to the evolution of metazoan recipients is rare.ResultsIn this study, based on sequence data accumulated to date, we used a unified method consisting of similarity search and phylogenetic analysis to detect horizontally transferred genes (HTGs) between prokaryotes and five insect species including Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Tribolium castaneum and Apis mellifera. Unexpectedly, the candidate HTGs were not detected in D. melanogaster, An. gambiae and T. castaneum, and 79 genes in Ap. mellifera sieved by the same method were considered as contamination based on other information. Consequently, 14 types of 22 HTGs were detected only in the silkworm. Additionally, 13 types of the detected silkworm HTGs share homologous sequences in species of other Lepidopteran superfamilies, suggesting that the majority of these HTGs were derived from ancient transfer events before the radiation of Ditrysia clade. On the basis of phylogenetic topologies and BLAST search results, donor bacteria of these genes were inferred, respectively. At least half of the predicted donor organisms may be entomopathogenic bacteria. The predicted biochemical functions of these genes include four categories: glycosyl hydrolase family, oxidoreductase family, amino acid metabolism, and others.ConclusionsThe products of HTGs detected in this study may take part in comprehensive physiological metabolism. These genes potentially contributed to functional innovation and adaptability of Lepidopteran hosts in their ancient lineages associated with the diversification of angiosperms. Importantly, our results imply that pathogens may be advantageous to the subsistence and prosperity of hosts through effective HGT events at a large evolutionary scale.

Dataset Information

Data on horizontally transferred genes in California two-spot octopus, Octopus bimaculoides.

Publications

Data on horizontally transferred genes in California two-spot octopus, <i>Octopus bimaculoides</i>.

Similar Datasets

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