Dataset Information

Transforming Growth Factor-β Protects against Inflammation-Related Atherosclerosis in South African CKD Patients.

ABSTRACT:

Background

Transforming growth factor-β (TGF-β) may inhibit the development of atherosclerosis. We evaluated serum levels of TGF-β isoforms concurrently with serum levels of endotoxin and various inflammatory markers. In addition, we determined if any association exists between polymorphisms in the TGF-β1 gene and atherosclerosis in South African CKD patients.

Methods

We studied 120 CKD patients and 40 healthy controls. Serum TGF-β1, TGF-β2, TGF-β3, endotoxin, and inflammatory markers were measured. Functional polymorphisms in the TGF-β1 genes were genotyped using a polymerase chain reaction-sequence specific primer method and carotid intima media thickness (CIMT) was assessed by B-mode ultrasonography.

Results

TGF-β isoforms levels were significantly lower in the patients with atherosclerosis compared to patients without atherosclerosis (p<0.001). Overall, TGF-β isoforms had inverse relationships with CIMT. TGF-β1 and TGF-β2 levels were significantly lower in patients with carotid plaque compared to those without carotid plaque [TGF-β1: 31.9 (17.2 - 42.2) versus 45.9 (35.4 - 58.1) ng/ml, p=0.016; and TGF-β2: 1.46 (1.30 - 1.57) versus 1.70 (1.50 - 1.87) ng/ml, p=0.013]. In multiple logistic regression, age, TGF-β2, and TGF-β3 were the only independent predictors of subclinical atherosclerosis in CKD patients [age: odds ratio (OR), 1.054; 95% confidence interval (CI): 1.003 - 1.109, p=0.039; TGF-β2: OR, 0.996; 95% CI: 0.994-0.999, p=0.018; TGF-β3: OR, 0.992; 95% CI: 0.985-0.999, p=0.029). TGF-β1 genotypes did not influence serum levels of TGF-β1 and no association was found between the TGF-β1 gene polymorphisms and atherosclerosis risk.

Conclusion

TGF-β isoforms seem to offer protection against the development of atherosclerosis among South African CKD patients.

SUBMITTER: Hassan MO

PROVIDER: S-EPMC6011064 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

Transforming Growth Factor-<i>β</i> Protects against Inflammation-Related Atherosclerosis in South African CKD Patients.

Hassan Muzamil Olamide MO Duarte Raquel R Dix-Peek Therese T Dickens Caroline C Naidoo Sagren S Vachiat Ahmed A Grinter Sacha S Manga Pravin P Naicker Saraladevi S

International journal of nephrology 20180606

<h4>Background</h4>Transforming growth factor-<i>β</i> (TGF-<i>β</i>) may inhibit the development of atherosclerosis. We evaluated serum levels of TGF-<i>β</i> isoforms concurrently with serum levels of endotoxin and various inflammatory markers. In addition, we determined if any association exists between polymorphisms in the <i>TGF-β1</i> gene and atherosclerosis in South African CKD patients.<h4>Methods</h4>We studied 120 CKD patients and 40 healthy controls. Serum TGF-<i>β</i>1, TGF-<i>β</i> ...[more]

PMID: 29977619

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