Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions.

ABSTRACT:

Background

Strawberry has received much attention due to its nutritional value, unique flavor, and attractive appearance. The availability of the whole genome sequence and multiple transcriptome databases allows the great possibility to explore gene functions, comprehensively. Gene expression profiles of a target gene can provide clues towards the understanding of its biological function. Quantitative real-time PCR (qRT-PCR) is a preferred method for rapid quantification of gene expression. The accuracy of the results obtained by this method requires the reference genes with consistently stable expression to normalize its data.

Results

In present study, the expression stability of seven candidate reference genes in diverse sample subsets of different tissues and fruit develo

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6013875 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature