Ontology highlight
ABSTRACT:
SUBMITTER: Pandey A
PROVIDER: S-EPMC6014917 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Pandey Alok A Pain Jayashree J Dziuba Nathaniel N Pandey Ashutosh K AK Dancis Andrew A Lindahl Paul A PA Pain Debkumar D
Cell chemical biology 20180426 6
In eukaryotes, mitochondria have been hypothesized to generate sulfur species required for tRNA thiolation in the cytosol, although no direct evidence thus far exists. Here we have detected these sulfur species, making use of our observation that isolated yeast cytosol alone is unable to thiolate tRNAs but can do so upon addition of mitochondria. Mitochondria were found to utilize the cysteine desulfurase Nfs1 to produce sulfur-containing species with masses ranging from 700 to 1,100 Da. Mitocho ...[more]