Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes.
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ABSTRACT:
SUBMITTER: Deng NN
PROVIDER: S-EPMC6016064 | biostudies-literature | 2018 Jun
REPOSITORIES: biostudies-literature
Publications
Macromolecularly Crowded Protocells from Reversibly Shrinking Monodisperse Liposomes.
Deng Nan-Nan NN Vibhute Mahesh A MA Zheng Lifei L Zhao Hui H Yelleswarapu Maaruthy M Huck Wilhelm T S WTS
Journal of the American Chemical Society 20180612 24
The compartmentalization of cell-free gene expression systems in liposomes provides an attractive route to the formation of protocells, but these models do not capture the physical (crowded) environment found in living systems. Here, we present a microfluidics-based route to produce monodisperse liposomes that can shrink almost 3 orders of magnitude without compromising their stability. We demonstrate that our strategy is compatible with cell-free gene expression and show increased protein produ ...[more]
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