Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC6016701 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Chen Jian J Zhao Zijun Z Chen Yangyijun Y Zhang Jie J Yan Lifu L Zheng Xiaocui X Liao Ming M Cao Weisheng W
Poultry science 20180701 7
Avian leukosis virus subgroup K (ALV-K) is an emerging ALV tumor virus of chickens. We developed a SYBR green-based real-time polymerase chain reaction (PCR) assay for the rapid and economical detection of ALV-K in chicken flocks. The assay was specific for ALV-K and did not cross-react with other ALV subgroup or avian influenza virus, Newcastle disease virus, or Marek's Disease virus. The method was 100 times more sensitive than conventional PCR and 10 times more sensitive than the enzyme-linke ...[more]