Dataset Information

High-resolution ?CT of a mouse embryo using a compact laser-driven X-ray betatron source.

ABSTRACT: In the field of X-ray microcomputed tomography (?CT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources is not practical for certain applications, and therefore the development of high-brightness laboratory-scale sources is crucial. We present here imaging of a fixed embryonic mouse sample using a compact laser-plasma-based X-ray light source and compare the results to images obtained using a commercial X-ray ?CT scanner. The radiation is generated by the betatron motion of electrons inside a dilute and transient plasma, which circumvents the flux limitations imposed by the solid or liquid anodes used in conventional electron-impact X-ray tubes. This X-ray source is pulsed (duration <30 fs), bright (>1010 photons per pulse), small (diameter <1 ?m), and has a critical energy >15 keV. Stable X-ray performance enabled tomographic imaging of equivalent quality to that of the ?CT scanner, an important confirmation of the suitability of the laser-driven source for applications. The X-ray flux achievable with this approach scales with the laser repetition rate without compromising the source size, which will allow the recording of high-resolution ?CT scans in minutes.

SUBMITTER: Cole JM

PROVIDER: S-EPMC6016801 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source.

Cole Jason M JM Symes Daniel R DR Lopes Nelson C NC Wood Jonathan C JC Poder Kristjan K Alatabi Saleh S Botchway Stanley W SW Foster Peta S PS Gratton Sarah S Johnson Sara S Kamperidis Christos C Kononenko Olena O De Lazzari Michael M Palmer Charlotte A J CAJ Rusby Dean D Sanderson Jeremy J Sandholzer Michael M Sarri Gianluca G Szoke-Kovacs Zsombor Z Teboul Lydia L Thompson James M JM Warwick Jonathan R JR Westerberg Henrik H Hill Mark A MA Norris Dominic P DP Mangles Stuart P D SPD Najmudin Zulfikar Z

Proceedings of the National Academy of Sciences of the United States of America 20180605 25

In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources is not practical for certain applications, and therefore the development of high-brightness laboratory-scale sources is crucial. We present here imaging of a fixed embryonic mouse sample using a compact laser-plasma-based X-ra ...[more]

PMID: 29871946

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Project description:This work aims to test accuracy and comparability of 3D models of human skeletal fibulae generated by clinical CT and laser scanner virtual acquisitions. Mesh topology, segmentation and smoothing protocols were tested to assess variation among meshes generated with different scanning methods and procedures, and to evaluate meshes-interchangeability in 3D geometric morphometric analysis. A sample of 13 left human fibulae were scanned separately with Revolution Discovery CT dual energy (0.625 mm resolution) and ARTEC Space Spider 3D structured light laser scanner (0.1 mm resolution). Different segmentation methods, including half-maximum height (HMH) and MIA-clustering protocols, were compared to their high-resolution standard generated with laser-scanner by calculating topological surface deviations. Different smoothing algorithms were also evaluated, such as Laplacian and Taubin smoothing. A total of 142 semilandmarks were used to capture the shape of both proximal and distal fibular epiphyses. After Generalized Procrustes superimposition, the Procrustes coordinates of the proximal and distal fibular epiphyses were used separately to assess variation due to scanning methods and the operator error. Smoothing algorithms at low iteration do not provide significant variation among reconstructions, but segmentation protocol may influence final mesh quality (0.09-0.24 mm). Mean deviation among CT-generated meshes that were segmented with MIA-clustering protocol, and laser scanner-generated ones, is optimal (0.42 mm, ranging 0.35-0.56 mm). Principal component analysis reveals that homologous samples scanned with the two methods cluster together for both the proximal and distal fibular epiphyses. Similarly, Procrustes ANOVA reveals no shape differences between scanning methods and replicates, and only 1.38-1.43% of shape variation is due to scanning device. Topological similarities support the comparability of CT- and laser scanner-generated meshes and validate its simultaneous use in shape analysis with potential clinical relevance. We precautionarily suggest that dedicated trials should be performed in each study when merging different data sources prior to analyses.

Dataset Information

High-resolution ?CT of a mouse embryo using a compact laser-driven X-ray betatron source.

Publications

High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source.

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