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Steroidal Saponins from Vernonia amygdalina Del. and Their Biological Activity.

ABSTRACT: In the present study, four new steroidal saponins, namely vernoniamyoside A-D (1-4), together with the two known steroidal saponins vernoamyoside D (5) and vernonioside B? (6) were isolated from the ethanol extract of leaves of the African medicinal plant Vernonia amygdalina Del. (Asteraceae). Their structures were demonstrated by spectral analyses along with 1D and 2D nuclear magnetic resonance (NMR) techniques and mass spectrometry (MS). The cytotoxicity of the compounds was also tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method on the cell lines Hela, MCF-7, BT-549 and MDA-MB-231. Vernoniamyoside A, vernoniamyoside B, and vernonioside B? showed cytotoxicity towards BT-549 cell lines. Vernoniamyoside C, vernoniamyoside D and vernoamyoside D showed different levels of cytotoxic activities.

SUBMITTER: Wang J

PROVIDER: S-EPMC6017044 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Steroidal Saponins from Vernonia amygdalina Del. and Their Biological Activity.

Wang Jing J Song Hua H Wu Xiaoxue X Zhang Shuyi S Gao Xuemin X Li Funan F Zhu Xuan X Chen Qing Q

Molecules (Basel, Switzerland) 20180305 3

In the present study, four new steroidal saponins, namely vernoniamyoside A-D (1-4), together with the two known steroidal saponins vernoamyoside D (5) and vernonioside B₂ (6) were isolated from the ethanol extract of leaves of the African medicinal plant Vernonia amygdalina Del. (Asteraceae). Their structures were demonstrated by spectral analyses along with 1D and 2D nuclear magnetic resonance (NMR) techniques and mass spectrometry (MS). The cytotoxicity of the compounds was also tested by the ...[more]

PMID: 29510543

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Vernonia amygdalina

Project description:Vernonia amygdalina Transcriptome

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Polyhydric Stigmastane-Type Steroids Derivative from Vernonia amygdalina and Their Anti-Neuroinflammatory Activity.

Project description:Vernonia amygdalina Del. is a traditional medicinal plant and vegetable originating from tropical Africa. The phytochemical investigation of V. amygdalina led to eight undescribed polyhydric stigmastane-type steroids, vernonin M-T (1-8). Their gross structures and stereochemistry were elucidated by HR-ESI-MS, 1D and 2D NMR spectra, X-ray diffraction, quantum chemical computation of the ECD spectrum, and the in situ dimolybdenum CD method. The anti-neuroinflammatory activity of the isolated compounds was performed in BV-2 microglia cells. As a result, compound 1 displayed a notable anti-neuroinflammatory effect via suppressing the LPS-induced IκB degradation and restricting the activation of the PI3K/AKT and p38 MAPK pathways.

| S-EPMC9506346 | biostudies-literature

Simultaneous study of antioxidant activity, DNA protection and anti-inflammatory effect of Vernonia amygdalina leaves extracts.

Project description:Vernonia amygdalina (VA) has been reported to have antioxidant potential; however, its DNA protection and anti-inflammatory properties remain unclear. We aimed to investigate whether aqueous (WEVAL) and alcoholic (EEVAL) VA extracts exert similar antioxidant, DNA protection and anti-inflammatory effects and attempted to explore the mechanism underlying the anti-inflammatory effects. These results demonstrated that WEVAL had greater polyphenolic and flavonoid contents, as well as a stronger reducing power, DPPH radical scavenging and DNA protective activity. Moreover, both extracts reduced lipopolysaccharide (LPS)-induced expression of COX-II, iNOS, pro-inflammatory factors, including NO, TNF-?, IL-1?, and IL-10. Compared with WEVAL, EEVAL was a more potent inflammatory inhibitor. Both extracts similarly inhibited LPS-induced MAPK (p38) and NF-?B expression. Our findings indicate that WEVAL and EEVAL have diverse antioxidant and anti-inflammatory effects. WEVAL had a stronger antioxidant and DNA protection activity; contrastingly, EEVAL had a stronger anti-inflammatory ability. The anti-inflammatory activity involves reduced pro-inflammatory cytokines through NF-?B down-regulation and MAPK inhibition. These results demonstrated that production of WEVAL and EEVAL from VA leaves may provide a safe and efficacious source of pharmaceutical applications, with antioxidant, DNA protective and anti-inflammation activities.

| S-EPMC7357738 | biostudies-literature

Antibacterial and Antioxidant Compounds from the Flower Extracts of Vernonia amygdalina.

Project description:Vernonia amygdalina is traditionally used in Ethiopia to treat various diseases. This prompted us to isolate bioactive compounds from the flowers of this plant. The CHCl3 extract after silica gel column chromatography has led to the isolation of two compounds identified as tricosane (1) and vernolide (2), while the acetone extract furnished isorhamnetin (3) and luteolin (4). The acetone extract and isorhamnetin significantly scavenged the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by 91.6 and 94%, respectively. It was also shown that the acetone extract and isorhamnetin inhibited lipid peroxidation by 74 and 80%, respectively. The extracts and isolated compounds were also evaluated for their antibacterial activity with the CHCl3 extract and vernolide showing strong activity against S. aureus with an inhibition zone of 21 and 19?mm, respectively. On the other hand, the acetone extract and isorhamnetin were active against all bacterial pathogens tested. The work presented herein has demonstrated that vernolide and isorhamnetin had antibacterial activity. The antioxidant activity displayed by the flowers of V. amygdalina is accounted to the presence of isorhamnetin. Therefore, the biological activities displayed by the extracts and isolated compounds from this plant corroborate the traditional uses of this plant by the local people against various diseases.

| S-EPMC5884303 | biostudies-literature

The complete chloroplast genome sequence of Vernonia amygdalina Delile.

Project description:Vernonia amygdalina Delile is widely used in folkloric medicine for the treatment of various diseases. In this study, the complete chloroplast genome of V. amygdalina Delile was reported, which was assembled and annotated base on genome high-throughput sequencing data. This work provided the clues for the taxonomy of the herb and the potential to utilize the chloroplast genome sequence as a new study target. The length of V. amygdalina Delile chloroplast genome was 153,133bp, with two single-copy regions, each has the length of 84,245bp and 13,152bp respectively. This region were separated by two inverted repeat regions with 27,868bp in length. It was predicted to consist of 131 genes in total, including 86 protein-coding genes, 37 tRNA genes, 8 rRNA genes with GC content at 37.68%. Phylogenetic analysis by RAxML (Random Axelerated Maximum Likelikhood) showed V. amygdalina Delile is closest to Sonchus webbii in Compositae.

| S-EPMC7995831 | biostudies-literature

Evaluation of the Antidiarrheal Activity of Hydromethanol Crude Extracts of Ruta chalepensis and Vernonia amygdalina in Mice.

Project description:Background:The herbal medicine practitioners in Ethiopia have used a wide range of medicinal plants as antidiarrheal agents. Among these, Ruta chalepensis and Vernonia amygdalina were claimed to have antidiarrheal activity in Ethiopian folklore medicine. Hence, the aim of the present study was to evaluate the antidiarrheal activity of the crude extracts of Ruta chalepensis and Vernonia amygdalina in mice. Methods:The crude extracts were obtained by cold maceration with 80% methanol, and its antidiarrheal activities were evaluated using a castor oil-induced diarrheal model. The test groups were treated with 100, 200, and 400?mg/kg body weight (bw) of the crude extract of each plant, while the positive controls and negative controls were given loperamide (3?mg/kg.bw) and 2% Tween 80 (10?ml/kg.bw), respectively. Results:In the castor oil-induced model, the crude extract of Ruta chalepensis (200 and 400?mg/kg.bw) significantly prolonged the onset of diarrhea in mice. Besides, it also showed a significant reduction in the frequency of stooling and weight of feces. Contrastingly, the crude extract of Vernonia amygdalina had a significant effect in delaying the onset of time of diarrhea and reduction of the frequency of stool and the weight of feces only at the maximum tested dose (400?mg/kg.bw). Conclusion:The present study demonstrated that the crude leaves extract of Ruta chalepensis (200 and 400?mg/kg.bw) and Vernonia amygdalina (400?mg/kg.bw) possessed significant antidiarrheal activity in the castor oil-induced diarrheal model.

| S-EPMC7174968 | biostudies-literature

Alpha-Glucosidase Inhibitory Activity of Saponins Isolated from Vernonia gratiosa Hance.

Project description:Species belonging to the Vernonia (Asteraceae), the largest genus in the tribe Vernonieae (consisting of about 1,000 species), are widely used in food and medicine. These plants are rich sources of bioactive sesquiterpene lactones and steroid saponins, likely including many as yet undiscovered chemical components. A phytochemical investigation resulted in the separation of three new stigmastane-type steroidal saponins (1 - 3), designated as vernogratiosides A-C, from whole plants of V. gratiosa. Their structures were elucidated based on infrared spectroscopy (IR), one-dimensional (1D) and two-dimensional nuclear magnetic resonance (2D NMR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and electronic circular dichroism analyses (ECD), as well as chemical reactivity. Molecular docking analysis of representative saponins with α-glucosidase inhibitory activity was performed. Additionally, the intended substances were tested for their ability to inhibit α-glucosidase activity in a laboratory setting. The results suggested that stigmastane-type steroidal saponins from V. gratiosa are promising candidate antidiabetic agents.

| S-EPMC10331943 | biostudies-literature

Neuroprotective mechanism of Vernonia amygdalina in a rat model of neurodegenerative diseases.

Project description:The global upsurge in the prevalence of neurodegenerative diseases in recent years has been associated with increase in toxic chemical exposure and release into the biosystem, having over 46.8 million people suffer dementia worldwide. This study focused on elucidating the neuroprotective mechanism of methanol leaf extract of Vernonia amygdalina (MLVA) in nitrobenzene-induced neurodegenerative disease in rats. Thirty aged male rats were sorted into five groups of six rats each. Group A received distilled water while 100 mg/kg bw of nitrobenzene was orally administered to groups (B to E) to induce neurodegeneration. Group B (disease control) was untreated, while Group C and D were treated with oral administration of 200 and 400 mg/kg bw of MLVA respectively and group E with vitamin E for 14 days. Locomotor behaviour was analysed using video-tracking software while the midbrain, cerebrum and cerebellum of the rats were processed for biochemical analyses. Results showed that treatment of nitrobenzene-induced neurodegenerative rats with MLVA significantly (p < 0.05) increase dopamine, GSH, antioxidant enzymes levels; and decrease acetylcholinesterase activity, biomarkers of inflammatory and oxidative stress level. Also, MLVA enhanced neurobehavioural and locomotor activities in all markers assessed. Taken together, neuroprotective mechanisms of MLVA can be linked to its antioxidant, acetylcholinesterase suppression, lipid peroxidation inhibition, anti-inflammatory and neurobehavioural restoring abilities.

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A novel East African monopartite begomovirus-betasatellite complex that infects Vernonia amygdalina.

Project description:The complete genomes of a monopartite begomovirus (genus Begomovirus, family Geminiviridae) and an associated betasatellite found infecting Vernonia amygdalina Delile (family Compositae) in Uganda were cloned and sequenced. Begomoviruses isolated from two samples showed the highest nucleotide sequence identity (73.1% and 73.2%) to an isolate of the monopartite begomovirus tomato leaf curl Vietnam virus, and betasatellites from the same samples exhibited the highest nucleotide sequence identity (67.1% and 68.2%) to vernonia yellow vein Fujian betasatellite. Following the current taxonomic criteria for begomovirus species demarcation, the isolates sequenced here represent a novel begomovirus species. Based on symptoms observed in the field, we propose the name vernonia crinkle virus (VeCrV) for this novel begomovirus and vernonia crinkle betasatellite (VeCrB) for the associated betasatellite. This is the first report of a monopartite begomovirus-betasatellite complex from Uganda.

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Green synthesis of silver nanoparticles using Vernonia amygdalina plant extract and its antimicrobial activities

Project description:The green nanoparticles synthesis method from leaves extract revealed full an economical, sustainable and eco-friendly method. In this study, the leaf extract of Vernonia amygdalina was as a reducing and capping agent for the synthesis of silver nanoparticles (AgNPs). M/DW binary solvent was selected for its relatively better extraction performance than methanol, ethanol, distilled water and ethanol/distilled water. Furthermore, the effect of solvent ratio of M/DW, precursor concentration, ratio of silver nitrate (AgNO3) to plant extract, temperature, time and pH on the synthesis of AgNPs was carried out. Greenly synthesized Agents was confirmed using UV–Vis spectroscopy and characterized by XRD and FT-IR. Besides, its antimicrobial activities were also evaluated using agar diffusion techniques. The UV–Vis spectra showed specific Surface Plasmon Resonance (SPR) absorption peaks between 411 nm and 430 nm which revealed the formation of AgNPs during the synthesis. The nanoparticle synthesis was further confirmed by XRD analysis. Phytochemical screening test and FT-IR analysis of V. amygdalina leaves extract revealed the existence of phenolic, Tannin, saponins and flavonoid groups, which capped the nanoparticles during the synthesis. The antibacterial activities of the synthesized AgNPs were evaluated against Gram-positive bacteria (S. pyogenes and S. aureus) and Gram-negative bacteria (E. coli and P. aeruginosa) and higher inhibition zones were observed.

| S-EPMC10293723 | biostudies-literature

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