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Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts.

ABSTRACT: This study was performed to evaluate the metabolite recovery from different extraction methods applied to Thymusalgeriensis aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 °C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.

SUBMITTER: Boutaoui N 

PROVIDER: S-EPMC6017170 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts.

Boutaoui Nassima N   Zaiter Lahcene L   Benayache Fadila F   Benayache Samir S   Carradori Simone S   Cesa Stefania S   Giusti Anna Maria AM   Campestre Cristina C   Menghini Luigi L   Innosa Denise D   Locatelli Marcello M  

Molecules (Basel, Switzerland) 20180220 2

This study was performed to evaluate the metabolite recovery from different extraction methods applied to <i>Thymus</i><i>algeriensis</i> aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min a  ...[more]

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