Ontology highlight
ABSTRACT:
SUBMITTER: Li Y
PROVIDER: S-EPMC6017996 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Li Yaoyao Y Wang Haoxin H Liu Yan Y Jiao Yujie Y Li Shanren S Shen Yuemao Y Du Liangcheng L
Angewandte Chemie (International ed. in English) 20180425 21
The biocontrol agent Lysobacter enzymogenes produces polycyclic tetramate macrolactams (PoTeMs), including the antifungal HSAF. To elucidate the biosynthesis of the cyclic systems, we identified eleven HSAF precursors/analogues with zero, one, two, or three rings through heterologous expression of the HSAF gene cluster. A series of combinatorial gene expression and deletion experiments showed that OX3 is the "gatekeeper" responsible for the formation of the first 5-membered ring from lysobactere ...[more]